EFFECTS OF BONE ASSOCIATED GROWTH-FACTORS ON DNA, COLLAGEN AND OSTEOCALCIN SYNTHESIS IN CULTURED FETAL-RAT CALVARIAE
EFFECTS OF BONE ASSOCIATED GROWTH-FACTORS ON DNA, COLLAGEN AND OSTEOCALCIN SYNTHESIS IN CULTURED FETAL-RAT CALVARIAE
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DOI：
10.1016/8756-3282(88)90037-3
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发表时间：
1988-01-01
期刊：
影响因子：
4.1
通讯作者：
LIAN, JB
中科院分区：
文献类型：
--
作者：
CANALIS, E;LIAN, JB
在骨和骨细胞培养中的研究表明，骨钙素的合成取决于成骨细胞的成熟度和1，25-二羟基维生素D3[1，25(OH)2D3]的存在。骨基质是丰富的生长因子来源，在骨形成中发挥作用，但它们对骨钙素合成的影响以及它们与1，25(OH)2D3的相互作用尚未被研究。胰岛素样生长因子I、碱性和酸性成纤维细胞生长因子、血小板衍生生长因子和转化生长因子。(TGF.beta)，是与骨基质相关的生长因子。在培养24 h的胎鼠颅骨细胞中，这些因素均能刺激DNA的[~3H]胸腺嘧啶核苷掺入，其作用不被1，25(OH)2D3所改变。IGF I和TGF.beta。1，25(OH)2D3对对照组、IGF I和TGF.β细胞的胶原合成均有抑制作用。治疗过的头盖骨。胰岛素样生长因子I、碱性成纤维细胞生长因子和碱性成纤维细胞生长因子可促进骨钙素合成1.5~2.5倍，但只有胰岛素样生长因子I与1，25(OH)2D3的刺激作用有协同作用。PDGF和TGF.beta。对骨钙素的合成无影响。总之，骨基质相关因子在骨培养中具有重要的促有丝分裂作用，但只有IGF I和FGFs刺激骨钙素的合成，与1，25(OH)2D3相比，这一作用幅度较小。
Studies in bone and bone cell cultures have shown that osteoclacin synthesis is dependent on the maturity of the osteoblast and the presence of 1,25-dihydroxyvitamin D3 [1,25(OH)2D3]. The bone matrix is a rich source of growth factors that play a role in bone formation, but their effects on osteocalcin synthesis and their interactions with 1,25(OH)2D3 have not been examined. Insulin-like growth factor I(IGF I), basic and acidic fibroblast growth factor (bFGF and aFGF), platelet-derived growth factor (PDGF) and transforming growth factor .beta. (TGF.beta.), are growth factors associated with the bone matrix. These factors were shown to stimulate [3H]thymidine incorporation into DNA in 24 h cultures of fetal rat calvariae, and their effect was not modified by 1,25(OH)2D3. IGF I and TGF.beta. stimulated [3H]proline incorporation into calvarial collagen while the other growth factors studied did not; 1,25(OH)2D3 inhibited collagen synthesis in control as well as in IGF I and TGF.beta. treated calvariae. IGF I, bFGF and aFGF stimulated osteocalcin synthesis 1.5 to 2.5 fold but only IGF I was synergistic with the stimulatory effect of 1,25(OH)2D3. PDGF and TGF.beta. had no effect on osteocalcin synthesis. In conclusion, bone matrix-associated factors have important mitogenic effects in bone cultures, but only IGF I and FGFs stimulate osteocalcin synthesis, an effect that is of small magnitude when compared to that of 1,25(OH)2D3.