Protein CheZ gain- and loss-of-function mutants
Protein CheZ gain- and loss-of-function mutants
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DOI：
10.1128/jb.178.21.6275-6280.1996
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发表时间：
1996-11-01
影响因子：
3.2
通讯作者：
Simon, MI
中科院分区：
文献类型：
--
作者：
Sanna, MG;Simon, MI
Bacterial chemotaxis results from the ability of flagellated bacteria to control the frequency of snitching between smooth-swimming and tumbling episodes in response to changes in concentration of extracellular substances. High levels of phosphorylated CheY protein are the intracellular signal for inducing the tumbling mode of swimming. The CheZ protein has been shown to control the level of phosphorylated CheY by regulating its rate of dephosphorylation, To identify functional domains in the CheZ protein, we made mutants by random mutagenesis of the cheZ gene and constructed a series of deletions. The map position and the in vivo and in vitro activity of the resulting gain- or loss-of-function mutant proteins define separate functional domains of the CheZ protein.
