Developmental regulation of pituitary adenylate cyclase-activating polypeptide and PAC1 receptor mRNA expression in the rat central nervous system
Developmental regulation of pituitary adenylate cyclase-activating polypeptide and PAC1 receptor mRNA expression in the rat central nervous system
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DOI：
10.1016/s0165-3806(99)00192-3
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发表时间：
2000-03-15
期刊：
影响因子：
--
通讯作者：
Proctor, MD
中科院分区：
文献类型：
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作者：
Jaworski, DM;Proctor, MD
随着大脑的发育，同质的有丝分裂活跃的祖细胞群体产生了成熟大脑中分子上不同的有丝分裂后细胞。这些祖细胞的分裂、生长停滞和分化之间的平衡无疑需要大量基因的激活。垂体腺苷环化酶激活多肽(PACAP)是血管活性肠肽(VIP)/促胰液素/胰高血糖素家族的成员。在神经系统内，PACAP已被证明可以刺激轴突生长，调节神经递质的产生和神经元的存活。这些不同的生物学作用是通过与两种类型的受体相互作用介导的，一种是PACAP选择性受体(PAC(1)-R)，另一种是与VIP和PACAP几乎等量作用的受体。由于一些证据表明PACAP作为一种神经营养因子，我们试图表征PACAP和PAC(1)-R在发育中的大鼠神经系统中的表达。PAC(1)-R在整个神经轴的脑室区域表达水平非常高。除了在增殖区丰富胚胎外，PAC(1)-R在成年中枢神经系统(CNS)的神经发生区，即嗅球的脑室下区和海马齿状回中保持表达。相反，PACAP主要在有丝分裂后的实质中表达。这种时间调控和细胞分布表明，PACAP通过与PAC(1)-R的相互作用，可能在哺乳动物的神经发生中发挥作用。(C)2000 Elsevier Science B.V.保留所有权利。
As the brain develops, a homogeneous population of mitotically active progenitors generates the molecularly heterogeneous post-mitotic cells of the mature brain. The balance between cell division, growth arrest and differentiation of these progenitors undoubtedly requires the activation of a vast array of genes. Pituitary adenylate cyclase-activating polypeptide (PACAP) is a member of the vasoactive intestinal polypeptide (VIP)/secretin/glucagon family. Within the nervous system, PACAP has been shown to stimulate neurite outgrowth, regulate neurotransmitter production and neuronal survival. These diverse biological actions are mediated through interaction with two types of receptors, a PACAP-selective receptor (PAC(1)-R) and receptors which interact almost equally with both VIP and PACAP. Since several lines of evidence suggest that PACAP acts as a neurotrophic factor, we sought to characterize PACAP and PAC(1)-R expression in the developing rat nervous system. The PAC(1)-R is expressed at very high levels in ventricular zones throughout the neuraxis. In addition to the embryonic enrichment in proliferative zones, PAC(1)-R expression is maintained in areas of neurogenesis in the adult central nervous system (CNS), namely, the subventricular zone of the olfactory bulb and hippocampal dentate gyrus. In contrast, PACAP is expressed primarily in the post-mitotic parenchyma. This temporal regulation and cellular distribution suggests that PACAP, through its interaction with the PAC(1)-R, may play a role in mammalian neurogenesis. (C) 2000 Elsevier Science B.V. All rights reserved.