Screening of Herbal-Based Bioactive Extract Against Carbapenem-Resistant Strain of Acinetobacter baumannii
Screening of Herbal-Based Bioactive Extract Against Carbapenem-Resistant Strain of Acinetobacter baumannii
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DOI：
10.1089/mdr.2015.0270
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发表时间：
2016-07-01
影响因子：
2.6
通讯作者：
Tiwari, Vishvanath
中科院分区：
文献类型：
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作者：
Tiwari, Monalisa;Roy, Ranita;Tiwari, Vishvanath
鲍曼不动杆菌被美国传染病学会归类为ESKAPE病原菌，与高发病率、高死亡率和高成本有关。这些细菌的高度获得性和内在耐药机制使得寻找一种合适的碳青霉烯类药物来替代碳青霉烯类药物成为当务之急。碳青霉烯是治疗不动杆菌感染的常用处方药。本研究对6种药用植物的乙醇提取物进行了植物化学筛选，并测定了它们对两株鲍曼不动杆菌(碳青霉烯类敏感菌株ATCC 19606和碳青霉烯类抗生素抗性菌株RS307)的抗菌活性。还测试了植物提取物与抗生素的协同作用。贝尔或宙斯盾含有单宁、苯酚、萜类、糖苷、生物碱、香豆素、类固醇和苯二酚。马达或红花的花中含有单宁、苯酚、萜类、糖苷、醌、蒽醌、花青素、香豆素和类固醇。苦瓜、姜黄和黄姜的叶和花对这两种细菌的生长均有抑制作用。单独使用抗生素显示出较小的抑制区，但与草药提取物一起使用时，它们显示出较大的抑制区。杏仁糖和亚胺培南对两株鲍曼不动杆菌的协同作用最好。从本研究中可以得出结论，苦杏仁叶和花的提取物具有最有效的抑菌活性。这些中草药提取物可用于筛选抗碳青霉烯类耐药鲍曼不动杆菌的活性化合物。
Acinetobacter baumannii is grouped in the ESKAPE pathogens by Infectious Disease Society of America, which is linked to high degree of morbidity, mortality, and increased costs. The high level of acquired and intrinsic resistance mechanisms of these bacteria makes it an urgent requirement to find a suitable alternative to carbapenem, a commonly prescribed drug for Acinetobacter infection. In this study, methanolic extracts of six medicinal plants were subjected to phytochemical screening and their antimicrobial activity was tested against two strains of A. baumannii (ATCC 19606, carbapenem-sensitive strain, and RS 307, carbapenem-resistant strain). Synergistic effect of the plant extracts and antibiotics was also tested. Bael or Aegle marmelos contains tannin, phenol, terpenoids, glycoside, alkaloids, coumarine, steroid, and quinones. Flowers of madar or Calotropis procera possess tannin, phenol, terpenoids, glycoside, quinone, anthraquinone, anthocyanin, coumarin, and steroid. An inhibitory growth curve was seen for both the bacterial strains when treated with A. marmelos, Curcuma longa, and leaves and flowers of C. procera. Antibiotics alone showed a small zone of inhibition, but when used with herbal extracts they exhibited larger zone of inhibition. Synergistic effect of A. marmelos and imipenem was the best against both the strains of A. baumannii. From this study, it can be concluded that extracts from A. marmelos and leaves and flowers of C. procera exhibited the most effective antibacterial activity. These herbal extracts may be used to screen the bioactive compound against the carbapenem-resistant strain of A. baumannii.