Myelin glycoproteins targeted by lead in the rodent model of prolonged exposure
Myelin glycoproteins targeted by lead in the rodent model of prolonged exposure
复制标题
DOI：
10.1016/j.fct.2007.10.024
复制
发表时间：
2008-03-01
影响因子：
4.3
通讯作者：
Rafalowska, U.
中科院分区：
文献类型：
--
作者：
Dabrowska-Bouta, B.;Struzynska, L.;Rafalowska, U.
这些实验的目的是辨别是否长期暴露于铅（Pb）的大鼠在水中，发生在典型的环境暴露，影响脑中的髓鞘的结构，髓鞘糖蛋白髓鞘相关糖蛋白（MAG）和髓鞘少突胶质细胞糖蛋白（MOG）的蛋白水平。在慢性铅中毒期间，髓鞘组分中的铅水平显著增加。发现长时间的铅暴露降低MAG和MOG的表达到相似的程度（对照值的80-85%）。此外，电子显微镜研究表明，轴突髓鞘的多层结构的解体，在结构上的变化的存在。这些形态学障碍铅中毒大鼠的髓鞘膜流动性的变化，采用脂肪酸自旋标记荧光分光光度法和电子顺磁共振（EPR）技术测量。在铅处理的大鼠中，观察到增强的膜流动性，如由膜的各向异性降低和有序参数降低所示。提示铅对成年大鼠脑内髓鞘的完整性有影响，而两种糖蛋白水平的降低可能是铅对髓鞘完整性影响的重要原因。(c)2007爱思唯尔有限公司保留所有权利。
The aim of these experiments was to discern whether prolonged exposure of rats to lead (Pb) in water, as occurs in typical environmental exposure, affects the structure of myelin in the brain, the protein levels of the myelin glycoproteins myelin associated glycoprotein (MAG) and myelin oligodendrocyte glycoprotein (MOG). During chronic lead intoxication, Pb levels in myelin fractions significantly increases. Prolonged lead exposure was found to decrease the expression of both MAG and MOG to a similar degree (80-85% of control values). Additionally, the electron microscopic studies have indicated the existence of structural changes in the axonal myelin sheath by revealing disintegration of its multilamellar structure. These morphological disturbances in Pb-intoxicated rats were paralleled by changes in myelin membrane fluidity as measured by spectrofluorometry and electron paramagnetic resonance (EPR) techniques employing a fatty acid spin label. In Pb-treated rats, enhanced membrane fluidity was observed, as indicated both by decreased anisotropy of the membrane and decreased order parameter. These results suggest that Pb influences the integrity of the myelin sheath in brain of adult rats and decreased level of both glycoproteins may significantly contribute to that effect. (c) 2007 Elsevier Ltd. All rights reserved.