Enrichment of oral mucosa and skin keratinocyte progenitor/stem cells.
Enrichment of oral mucosa and skin keratinocyte progenitor/stem cells.
复制标题
富集口腔粘膜和皮肤角质形成细胞祖细胞/干细胞。
DOI：
10.1007/978-1-62703-330-5_23
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发表时间：
2013
期刊：
影响因子：
--
通讯作者：
Feinberg,StephenE
中科院分区：
文献类型：
--
作者：
Izumi,Kenji;Marcelo,CynthiaL;Feinberg,StephenE
人口腔粘膜/皮肤角质形成细胞祖细胞/干细胞的分离对于再生上皮组织以治疗口腔粘膜/皮肤缺陷具有重要的临床意义。研究人员尝试使用细胞标记、快速粘附 IV 型胶原蛋白和其他方法来分离角质形成细胞祖细胞/干细胞群。在这方面，角质形成细胞祖细胞/干细胞的具体特征之一是这些细胞具有比分化细胞更小的直径。本章描述了我们实验室在不含动物源产品的化学成分确定的培养系统中建立原代人类口腔粘膜和皮肤角质形成细胞的方法。我们在 FDA 批准的人体临床试验中使用了这些细胞，该试验涉及口内移植离体产生的口腔粘膜，相当于增加牙齿周围的角化组织。我们还提供了两种关于如何使用物理标准、细胞大小、使用细胞分选仪和串行过滤系统对角质形成细胞进行分选的方案。
The isolation of human oral mucosa/skin keratinocytes progenitor/stem cells is clinically important to regenerate epithelial tissues for the treatment of oral mucosa/skin defects. Researchers have attempted to isolate a keratinocyte progenitor/stem cell population using cell markers, rapid adherence to collagen type IV, and other methods. In this regard, one of the specific characteristics of keratinocyte progenitor/stem cells is that these cells have a smaller diameter than differentiated cells. This chapter describes methods used in our laboratory to set up primary human oral mucosa and skin keratinocytes in a chemically defined culture system devoid of animal derived products. We utilized the cells in a FDA-approved human clinical trial that involved the intraoral grafting of an ex vivo produced oral mucosa equivalent to increase keratinized tissue around teeth. We also provide two protocols on how to sort keratinocytes using physical criterion, cell size, using a cell sorter and a serial filtration system.