Polymorphisms in the nine polymorphic membrane proteins of Chlamydia trachomatis across all serovars:: Evidence for serovar Da recombination and correlation with tissue tropism
Polymorphisms in the nine polymorphic membrane proteins of Chlamydia trachomatis across all serovars:: Evidence for serovar Da recombination and correlation with tissue tropism
复制标题
DOI：
10.1128/jb.188.1.275-286.2006
复制
发表时间：
2006-01-01
影响因子：
3.2
通讯作者：
Dean, D
中科院分区：
文献类型：
--
作者：
Gomes, JP;Nunes, A;Dean, D
沙眼衣原体是一种细胞内细菌，可引起眼部、呼吸道和性传播疾病。基因组包含一个9成员的多态性膜蛋白（Pmp）家族，该家族是衣原体目成员所特有的。对18种参考血清学变体（血清型）和基因变体Ja的整个pmp基因家族进行基因组和分子分析，以鉴定区分衣原体疾病组的特定基因和蛋白质区域。所有血清型之间的平均遗传距离从0.1%的pmpA到7.0%的pmpF。性病淋巴肉芽肿（LGV）血清型是最密切相关的pmp基因，也是最分歧的，相比，眼部和非LGV泌尿生殖系统疾病组。系统发育重建表明，六个9 pmp基因（不是pmpA，pmpD，或pmpE），血清型聚类的基础上组织嗜性。全球最成功的血清型，E和F，聚类远离泌尿生殖系统组的五个pmp基因。这些pmp基因可能赋予生物学优势，可能促进E和F的感染和传播。令人惊讶的是，血清型Da聚类与眼组从pmPE到pmPI，这是位于一起的染色体，提供了统计学上显着的证据，基因组间重组和收购的遗传组成，可以假设扩大宿主细胞范围的血清型Da。我们还确定了不同的结构域的pmPE，pmpF，和pmPH的取代集中，并与特定的疾病组。因此，我们的数据表明可能的结构或功能的作用，可能不同的pmp基因在促进抗原多态性和/或不同的粘附受体，可能参与免疫逃避和差异组织嗜性。
Chlamydia trachomatis is an intracellular bacterium responsible for ocular, respiratory, and sexually transmitted diseases. The genome contains a nine-member polymorphic membrane protein (Pmp) family unique to members of the order Chlamydiales. Genomic and molecular analyses were performed for the entire pmp gene family for the 18 reference serological variants (serovars) and genovariant Ja to identify specific gene and protein regions that differentiate chlamydial disease groups. The mean genetic distance among all serovars varied from 0.1% for pmpA to 7.0% for pmpF. Lymphogranuloma venereum (LGV) serovars were the most closely related for the pmp genes and were also the most divergent, compared to ocular and non-LGV urogenital disease groups. Phylogenetic reconstructions showed that for six of nine pmp genes (not pmpA,pmpD, or pmpE), the serovars clustered based on tissue tropism. The most globally successful serovars, E and F, clustered distantly from the urogenital group for five pmp genes. These pmp genes may confer a biologic advantage that may facilitate infection and transmission for E and F. Surprisingly, serovar Da clustered with the ocular group from pmpE to pmpI, which are located together in the chromosome, providing statistically significant evidence for intergenomic recombination and acquisition of a genetic composition that could hypothetically expand the host cell range of serovar Da. We also identified distinct domains for pmpE, pmpF, and pmpH where substitutions were concentrated and associated with a specific disease group. Thus, our data suggest a possible structural or functional role that may vary among pmp genes in promoting antigenic polymorphisms and/or diverse adhesions-receptors that may be involved in immune evasion and differential tissue tropism.