Enzymatic N-terminal addition of noncanonical amino acids to peptides and proteins
Enzymatic N-terminal addition of noncanonical amino acids to peptides and proteins
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DOI：
10.1002/cbic.200700605
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发表时间：
2008-01-15
期刊：
影响因子：
3.2
通讯作者：
Tirrell, David A.
中科院分区：
文献类型：
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作者：
Connor, Rebecca E.;Piatkov, Konstantin;Tirrell, David A.
方法叠氮诺亮氨酸-生物素（12）的合成：将叠氮诺亮氨酸（12.65 mg, 0.073 mmol）和pfp -生物素（30.1 mg, 0.073 mmol）溶于1.5 mL的二甲甲酰胺、二甲基亚砜和甲醇的9:1:5混合物中。加入四等量的三乙胺，室温搅拌反应过夜。在C18 Sep-Pak色谱柱（Waters）上使用从0.1%三氟乙酸到100%乙腈的逐步梯度纯化该反应。将含有所需产物的馏分冻干为6.8 mg的蓬松白色粉末（收率为23.4%）。ESI MS (m/z): 398.9
Methods Synthesis of azidonorleucine-biotin (12): Azidonorleucine (12.65 mg, 0.073 mmol) and PFP-biotin (30.1 mg, 0.073 mmol) were dissolved in 1.5 mL of a 9: 1: 5 mixture of dimethylformamide, dimethylsulfoxide and methanol. Triethylamine (4 equiv) was added and the reaction stirred overnight at room temperature. The reaction was purified on a C18 Sep-Pak column (Waters) using a stepwise gradient from 0.1% trifluoroacetic acid to 100% acetonitrile. The fractions containing the desired product were lyophilized to 6.8 mg of a fluffy white powder (23.4% yield). ESI MS (m/z): 398.9