FURTHER DESCRIPTION OF THE LY-5 SYSTEM
FURTHER DESCRIPTION OF THE LY-5 SYSTEM
复制标题
DOI：
10.1007/bf01570435
复制
发表时间：
1979-01-01
期刊：
影响因子：
3.2
通讯作者：
TRIGLIA, D
中科院分区：
文献类型：
--
作者：
SCHEID, MP;TRIGLIA, D
借助于Ly-5同源小鼠品系和一种额外的血清学技术-PA-SRBC[葡萄球菌蛋白A-绵羊红细胞]花环试验，重新评估了Ly-5同种异体抗原系统，这是以前没有应用于Ly-5的。在PA-SRBC检测中，PA偶联的SRBC与抗体包被的细胞反应形成玫瑰花环。PA-SRBC法检测Ly-5的敏感性高于细胞毒法，但胸腺细胞除外，后者对Ly-5的检测更为有效。使用这两种分析方法可以更全面地了解Ly-5系统。显然，Ly-5在大多数造血系细胞上都有表达，但到目前为止还没有在其他细胞上表达。Ly-5+细胞群包括所有已知的T[胸腺来源]细胞、原胸腺细胞、B[骨髓来源]细胞、髓系细胞和单核巨噬细胞类型以及自然杀伤细胞。红细胞和前红细胞为Ly-5-，但分化较低的红细胞的Ly-5表型尚不确定。通过用Ly-5同基因骨髓恢复致死照射小鼠的辐射嵌合体，肝和肾的弱Ly-5+表型是由于移行的循环细胞所致。肿瘤的Ly-5表型符合这种Ly-5组织表达方案。Ly-5+肿瘤包括40多例白血病、浆细胞瘤、表面免疫球蛋白阴性的转化等价物：LYB-2+早期B细胞、1个肥大细胞瘤、1个单核细胞系和3个与巨噬细胞相关的系。其他检测的肿瘤，肉瘤，自发性乳腺癌，畸胎癌和神经母细胞瘤，是Ly-5-。
The ly-5 system of alloantigens was reevaluated with the aid of an Ly-5 congenic mouse strain and an additional serological technique, the PA-SRBC [staphylococcal protein A-sheep red blood cells] rosette assay, not previously applied to Ly-5. In the PA-SRBC assay, SRBC to which PA was coupled react with antibody-coated cells to form rosettes. The PA-SRBC assay was more sensitive than the cytotoxicity assay in detecting Ly-5 on all cell types tested, with the exception of thymocytes, on which Ly-5 was more efficiently demonstrable by the latter test. Use of both assays gave a more complete picture of the Ly-5 system. Evidently, Ly-5 is expressed on most cells of the hematopoietic lineage, but on no other cells so far tested. The Ly-5+ cell population includes all known sets of T [thymus-derived] cells, prothymocytes, sets of B [bone marrow-derived] cells, cells of myeloid and monocyte-macrophage types and natural killer cells. Erythrocytes and proerythroblasts are Ly-5-, but the Ly-5 phenotype of less differentiated erythrocytic cells is uncertain. By radiation chimeras, made by restoring lethally irradiated mice with Ly-5 congenic bone marrow, the weakly Ly-5+ phenotype of liver and of kidney was due to immigrant circulating cells. Ly-5 phenotypes of tumors conform to this scheme of Ly-5 tissue representation. The Ly-5+ tumors included more than 40 leukemias tested, plasmacytomas, putative transformed equivalents of sIg- [surface immunoglobulin negative]:Lyb-2+ early B cells, a mastocytoma, a monocytic line and 3 lines related to the macrophage. Other tumors tested, a sarcoma, a spontaneous mammary carcinoma, a teratocarcinoma and a neuroblastoma, were Ly-5-.