Development of real-time PCR assayusing Taq Man probe for specific and quantitative detection of Rosellinia necatrix in plant and soil
Development of real-time PCR assayusing Taq Man probe for specific and quantitative detection of Rosellinia necatrix in plant and soil
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开发使用 Taq Man 探针的实时 PCR 检测方法，用于植物和土壤中 Rosellinia necatrix 的特异性和定量检测
DOI：
10.1007/s10327-012-0366-x
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发表时间：
2012
影响因子：
1.2
通讯作者：
H
中科院分区：
文献类型：
--
作者：
Shishido;M.;Kubota;I.;Nakamura;H
我们开发了实时荧光PCR检测TaqMan探针检测和定量Rosellinia necatrix，在许多植物物种的白色根腐病的病原体。两套PCR引物和TaqMan探针的检测限可低至1 fg的模板DNA。利用TaqMan探针进行实时荧光定量PCR检测，我们能够定量R。necatrixDNA在日本梨自然发病的根和人工感染的土壤样品。虽然新的检测方法不足以用于自然感染的土壤样品，巢式PCR程序提高了新的检测方法的可检测性。
We developed real-time PCR assays using TaqMan probes to detect and quantifyRosellinia necatrix, the causal agent of white root rot in many plant species. Two sets of PCR primers and TaqMan probe indicated that their detection limits could be as low as 1 fg of template DNA. Using the real-time PCR assays with the TaqMan probes, we were able to quantifyR. necatrixDNA in naturally diseased roots of Japanese pear and in artificially infested soil samples. Although the new assays were inadequate for use with naturally infested soil samples, nested PCR procedures improved the detectability of the new assays.