Multiple pathways for regulation of σS (RpoS) stability in Escherichia coli via the action of multiple anti-adaptors
Multiple pathways for regulation of σS (RpoS) stability in Escherichia coli via the action of multiple anti-adaptors
复制标题
DOI：
10.1111/j.1365-2958.2008.06146.x
复制
发表时间：
2008-04-01
影响因子：
3.6
通讯作者：
Gottesman, Susan
中科院分区：
文献类型：
--
作者：
Bougdour, Alexandre;Cunning, Christofer;Gottesman, Susan
sigma（S）是大肠杆菌和沙门氏菌的固定相sigma因子，在多个水平上受到调节。sigma（S）蛋白在指数生长期间是不稳定的，并且在稳定期期间和各种应力处理之后是稳定的。降解需要ClpXP蛋白酶和衔接子RssB两者。小的抗适应蛋白PARP是响应于磷酸盐饥饿而产生的，并与RssB相互作用，在这种应激条件下引起sigma（S）稳定。在某些但不是所有的饥饿条件下，σ（S）稳定化所必需的，这表明存在其他的反衔接蛋白。我们在这里报告的识别新的监管机构的西格玛（S）的稳定性，重要的其他压力条件下。镁饥饿期间RssB活性的抑制剂）和DNA损伤后RssB活性的抑制剂）在体内和体外均抑制σ（S）蛋白水解。我们的研究结果表明，多种抗接头蛋白允许通过在各种应激条件下调节RssB活性来调节sigma（S）稳定性。
sigma(S), the stationary phase sigma factor of Escherichia coli and Salmonella, is regulated at multiple levels. The sigma(S) protein is unstable during exponential growth and is stabilized during stationary phase and after various stress treatments. Degradation requires both the ClpXP protease and the adaptor RssB. The small antiadaptor protein IraP is made in response to phosphate starvation and interacts with RssB, causing sigma(S) stabilization under this stress condition. IraP is essential for sigma(S) stabilization in some but not all starvation conditions, suggesting the existence of other anti-adaptor proteins. We report here the identification of new regulators of sigma(S) stability, important under other stress conditions. IraM (inhibitor of RssB activity during Magnesium starvation) and IraD (inhibitor of RssB activity after DNA damage) inhibit sigma(S) proteolysis both in vivo and in vitro. Our results reveal that multiple anti-adaptor proteins allow the regulation of sigma(S) stability through the regulation of RssB activity under a variety of stress conditions.