Off-season flowering and expression of flowering-related genes during floral bud differentiation of rabbiteye blueberry in a subtropical climate
Off-season flowering and expression of flowering-related genes during floral bud differentiation of rabbiteye blueberry in a subtropical climate
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亚热带兔眼蓝莓反季节开花及花芽分化过程中开花相关基因的表达
DOI：
10.1016/j.scienta.2022.111458
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发表时间：
2022
影响因子：
4.3
通讯作者：
Li Kuo-Tan
中科院分区：
文献类型：
--
作者：
Omori Masafumi;Cheng Chieh-Chi;Hsu Fu-Chiun;Chen Shiang-Jiuun;Yamane Hisayo;Tao Ryutaro;Li Kuo-Tan
在亚热带气候下栽培的兔眼蓝莓（Vaccinium virgatumAiton）通常在茎尖上显示秋季开花，这为淡季浆果生产提供了潜力。本研究旨在通过田间观察和基因表达分析，阐明反季节开花的机理。2018-2020年的田间试验表明，顶端开花主要发生在非终止的茎尖，表明营养生长状态是顶端花发育所必需的。顶芽的显微结构表明，茎尖的营养生长与顶花序同时发生。为了了解反季节开花的分子基础，开花相关基因的表达，对兔眼蓝莓‘NTU 031’和‘Austin’的VcFT、VcCEN和VcCOL 5进行了分析。虽然这两个品种的大部分枝条在10月继续营养生长，'NTU 031'表现出强烈的反季节顶端开花从11月下旬，而大多数枝条的'奥斯汀'终止与顶端生长停止。在“NTU 031”中，随着花分生组织的发育，VcFT表达在10月中旬增加，而VcCEN和VcCOL 5表达下降。另一方面，“奥斯汀”在这些基因的表达中没有表现出任何明显的波动。结果表明，兔眼蓝莓秋季顶开花与VcFT的转录水平呈正相关，与VcCEN和VcCOL 5的表达水平呈负相关。
Rabbiteye blueberries (Vaccinium virgatumAiton) cultivated in subtropical climates often display autumn flowering on shoot apices, which offers a potential for off-season berry production. The aim of this study was to elucidate the mechanism of off-season flowering through field observation and gene expression analysis. Field trials from 2018-2020 indicated that apical blooming occurred mainly in non-terminated shoot tips, suggesting that vegetative growing status was necessary for the development of apical flowers. Microstructure of apical buds showed that vegetative growth on shoot tip and apical inflorescence developed simultaneously. To understand the molecular basis of off-season flowering, expression of flowering-related genes, i.e.,VcFT, VcCEN,andVcCOL5, in rabbiteye blueberry ‘NTU031’ and ‘Austin’ were analyzed. Although most shoots of the two cultivars continued vegetative growth in October, ‘NTU031’ showed intensive off-season apical flowering from late November while most shoots of ‘Austin’ terminated with apical growth cessation. In ‘NTU031’,VcFTexpression increased in mid-October with floral meristem development whereasVcCENandVcCOL5expression decreased. On the other hand, ‘Austin’ did not show any significant fluctuation in the expression of these genes. The results suggested that autumn apical flowering in rabbiteye blueberries was positively correlated with the transcription ofVcFTbut negatively correlated with the expression ofVcCENandVcCOL5.