GmCOL1a and GmCOL1b Function as Flowering Repressors in Soybean Under Long-Day Conditions
GmCOL1a and GmCOL1b Function as Flowering Repressors in Soybean Under Long-Day Conditions
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GmCOL1a 和 GmCOL1b 在长日照条件下作为大豆开花抑制剂
DOI：
10.1093/pcp/pcv152
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发表时间：
2015-12-01
影响因子：
4.9
通讯作者：
Kong, Fanjiang
中科院分区：
文献类型：
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作者：
Cao, Dong;Li, Ying;Kong, Fanjiang
CONSTANS （CO）在拟南芥光周期响应机制中起核心作用。然而，豆科植物CO基因在控制开花中的作用尚不清楚。本研究利用近等基因系（near-isogenic lines, NILs）分析了E1、E2和GmCOL1a/1b的表达模式，并进一步分析了gmcol1b突变体和GmCOL1a过表达植物的开花相关基因。我们的数据显示，E3和E4都上调了E1的表达，且E3对E1的影响大于E4对E1的影响。E2被E3和E4上调，E1下调。GmCOL1a/1b被E1、E2、E3和E4上调。虽然GmCOL1a/1b的时空表达模式与AtCOL2比AtCO更相似，但在长日照条件下，gmcol1b突变体比野生型植物开花时间更早，且在长日照或自然条件下，GmCOL1a过表达导致开花时间晚。此外，LD条件下gmcol1a过表达植物的GmFT2a/5a、E1和E2均下调。由于E1/2影响GmCOL1a的表达，反之亦然，我们得出结论，这些基因可能作为负反馈回路的一部分，GmCOL1a/b基因可能在LD条件下大豆光周期开花中起抑制作用。
CONSTANS (CO) has a central role in the photoperiod response mechanism in Arabidopsis. However, the functions of legume CO genes in controlling flowering remain unknown. Here, we analyze the expression patterns of E1, E2 and GmCOL1a/1b using near-isogenic lines (NILs), and we further analyze flowering-related genes in gmcol1b mutants and GmCOL1a-overexpressing plants. Our data showed that both E3 and E4 up-regulate E1 expression, with the effect of E3 on E1 being greater than the effect of E4 on E1. E2 was up-regulated by E3 and E4 but down-regulated by E1. GmCOL1a/1b were up-regulated by E1, E2, E3 and E4. Although the spatial and temporal patterns of GmCOL1a/1b expression were more similar to those of AtCOL2 than to those of AtCO, gmcol1b mutants flowered earlier than wild-type plants under long-day (LD) conditions, and the overexpression of GmCOL1a caused late flowering under LD or natural conditions. In addition, GmFT2a/5a, E1 and E2 were down-regulated in GmCOL1a-overexpressing plants under LD conditions. Because E1/2 influences the expression of GmCOL1a, and vice versa, we conclude that these genes may function as part of a negative feedback loop, and GmCOL1a/b genes may serve as suppressors in photoperiodic flowering in soybean under LD conditions.