Isolation and characterization of soluble sulfated polysaccharide from the red seaweed Gracilaria cornea
Isolation and characterization of soluble sulfated polysaccharide from the red seaweed Gracilaria cornea
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DOI：
10.1016/s0144-8617(02)00006-1
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发表时间：
2002-09-01
影响因子：
11.2
通讯作者：
de Paula, RCM
中科院分区：
文献类型：
--
作者：
Melo, MRS;Feitosa, JPA;de Paula, RCM
研究了巴西红龙须菜（Gracilaria cornea）可溶性硫酸多糖的组成、结构和流变学性质。测定了琼脂胶产率、特性粘度、单糖组成、硫酸根和阳离子含量以及分子量。多糖主要成分为3，6-脱水半乳糖（24.7%）和半乳糖（64.6%）。此外，还检测到少量组分，如6-O-甲基-半乳糖（8.5%）、葡萄糖（1.5%）、木糖（0.7%）和硫酸化基团（4.8%）。对傅里叶变换红外光谱法和微量元素分析法测定的硫酸根含量进行了比较。从C-13 NMR和FT-IR的数据提供了证据的硫酸化的C-4和C-6的半乳糖。在琼脂胶体中检测到钠、钙、镁和钾阳离子。在相同的实验条件下，特性粘度低于琼脂的典型值。在1.5、2.0和3.0%（w/v）水溶液中没有观察到凝胶化，甚至通过冷却至4 ℃。凝胶渗透色谱法显示两个主要的多糖组分M-pk 7.4 × 10（4）和1.8 × 10（4）g/mol，以及次要组分M-pk 2.1 × 10（6）g/mol，可能是蛋白质-多糖复合物。(C)2002年爱思唯尔科学有限公司保留所有权利。
The composition, structure and rheological properties of a soluble sulfated polysaccharide from Gracilaria cornea (Brazilian red marine alga) were investigated. Agarocolloid yield, intrinsic viscosity, monosaccharide composition, sulfate and cation content as well as molecular weight were determined. The main polysaccharide components were 3,6-anhydrogalactose (24.7%) and galactose (64.6%). In addition, minor components such as 6-O-methyl-galactose (8.5%), glucose (1.5%), xylose (0.7%) and sulfated groups (4.8%) were detected. Comparison between sulfate contents determined by Fourier transform IR (FT-IR) spectroscopy and microelemental analysis was made. Data from C-13 NMR and FT-IR provided evidence of sulfation in C-4 and C-6 of galactose. Sodium, calcium, magnesium and potassium cations were detected in the agarocolloid. The intrinsic viscosities were lower than typical values for agar in the same experimental conditions. No gelation in 1.5, 2.0 and 3.0% (w/v) aqueous solution was observed, even by cooling up to 4 degreesC. Gel permeation chromatography indicated two major polysaccharide fractions Of M-pk 7.4 x 10(4) and 1.8 x 10(4) g/mol and a minor fraction of M-pk 2.1 x 10(6) g/mol, probably a protein-polysaccharide complex. (C) 2002 Elsevier Science Ltd. All fights reserved.