Controlling the adsorption and reactivity of DNA on gold
Controlling the adsorption and reactivity of DNA on gold
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DOI：
10.1021/la035116y
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发表时间：
2003-12-09
期刊：
影响因子：
3.9
通讯作者：
Daube, SS
中科院分区：
文献类型：
--
作者：
Aqua, T;Naaman, R;Daube, SS
由于它的编码性质，它可以经历许多化学和酶操作，以及它的相对稳定性，DNA正被用作细胞外的支架和构建块。DNA连接到固体表面的方式是技术进步的核心，例如DNA芯片和生物传感器。人们的愿望是以预先设计的方式将DNA连接到给定的表面，并根据任何设备规格进行定制。在这项工作中，DNA分子被特异性地吸附在金的表面。通过一种新的方法来控制吸附的特异性，该方法首先用不同程度的疏水层(C-18-SH)封闭金表面，然后吸附硫代化的DNA。这项技术既适用于短DNA链，也适用于长DNA链。我们发现，硫代化的短DNA在连接反应中的反应性由于烷基硫醇层的存在而提高了一个数量级以上。由于C-18-SH层的疏水性和绝缘性，这种封闭方法对电子测量是有利的。
Due to its coding nature, the many chemical and enzymatic manipulations that it can undergo, and its relative stability, DNA is being used as a scaffold and a building block outside the cellular context. The mode by which the DNA is connected to a solid surface is in the heart of technological advancements, such as DNA chips and biosensors. The desire is to connect the DNA to a given surface in a predesigned manner, tailored to any device specifications. In this work, DNA molecules were adsorbed specifically on gold surfaces. The specificity of the adsorption was controlled by a novel approach, in which the gold surface was first blocked with a hydrophobic layer (C-18-SH) to various extents, followed by the adsorption of thiolated DNA. The technique was applied both for short and for long strands of DNA. We show that the reactivity of the thiolated short DNA in a ligation reaction is enhanced by more than an order of magnitude by the presence of the alkylthiol layer. Due to the hydrophobic and insulating nature of the C-18-SH layer, this blocking method is advantageous for electronic measurements.