Cyclic acetal hydrogel system for bone marrow stromal cell encapsulation and osteodifferentiation
Cyclic acetal hydrogel system for bone marrow stromal cell encapsulation and osteodifferentiation
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DOI：
10.1002/jbm.a.31640
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发表时间：
2008-09-01
影响因子：
4.9
通讯作者：
Fisher, John P.
中科院分区：
文献类型：
--
作者：
Betz, Martha W.;Modi, Parth C.;Fisher, John P.
已经提出了许多用于将骨髓基质细胞（BMSC）封装在可降解水凝胶内的系统。在这里，我们使用由5-乙基-5-(羟甲基)-β,β-二甲基-1,3-二恶烷-2-乙醇二丙烯酸酯(EHD)和聚(乙二醇)二丙烯酸酯(PEGDA)形成的新型环状缩醛基生物材料。基于环状缩醛的水凝胶可能是优选的，因为环状缩醛水解降解为二醇和羰基化合物作为主要降解产物，与其他广泛研究的聚合物不同，这可能不会影响局部酸度。 EHD单体和PEGDA聚合物可以通过过硫酸铵(APS)和N,N,N',N'-四甲基乙二胺(TEMED)体系引发的自由基聚合制备成EH-PEG水凝胶。这项工作的目的是确定 EH-PEG 水凝胶制造中使用的成分以及 EH-PEG 水凝胶是否允许 BMSC 活力、代谢活性和骨分化。暴露于相关浓度的引发剂系统 (10-20 mM) 30 分钟、1 小时和 3 小时后评估细胞活力和代谢活性。在短暂暴露于引发剂系统以模拟封装过程后，通过碱性磷酸酶和骨钙素表达来评估骨分化。最后，在封装后和在 EH-PEG 水凝胶内培养 7 天后立即评估细胞活力。结果表明，APS-TEMED 引发剂系统对 BMSC 的代谢活性和活力影响最小，并且骨分化没有受到显着影响。此外，封装的 BMSC 在 EH-PEG 水凝胶中保持活力 7 天。这项工作表明，EH-PEG 水凝胶是 BMSC 封装和骨分化的可行选择。 (c) 2007 年 Wiley 期刊公司。
Many systems have been proposed for the encapsulation of bone marrow stromal cells (BMSCs) within degradable hydrogels. Here, we use a novel cyclic acetal-based biomaterial formed from 5-ethyl-5-(hydroxymethyl)-beta,beta-dimethyl-1,3-dioxane-2-ethanol diacrylate (EHD) and poly(ethylene glycol) diacrylate (PEGDA). A cyclic acetal-based hydrogel may be preferred as cyclic acetals hydrolytically degraded into diols and carbonyls as primary degradation products, which may not affect local acidity, unlike other widely investigated polymers. The EHD monomer and PEGDA polymer may be fabricated into a EH-PEG hydrogel by radical polymerization initiated by the ammonium persulfate (APS) and N,N,N',N'-tetramethylethylenediamine (TEMED) system. The objective of this work is to determine whether the components utilized in the fabrication of EH-PEG hydrogels as well as the EH-PEG hydrogels permit BMSC viability, metabolic activity, and osteodifferentiation. Cell viability and metabolic activity were assessed after 30 min, 1 h, and 3 h of exposure to pertinent concentrations of the initiator system (10-20 mM). Osteodifferentiation was assessed by alkaline phosphatase and osteocalcin expression after a short exposure to the initiator system to simulate the encapsulation process. Lastly, cell viability was assessed immediately after encapsulation and after 7 days of culture within the EH-PEG hydrogels. results indicate that the metabolic activity and viability of BMSCs are minimally affected, and that osteodifferentiation is not significantly affected by the APS-TEMED initiator system. Also, encapsulated BMSCs maintained viability within EH-PEG hydrogels for 7 days. This work demonstrates, that the EH-PEG hydrogel is a viable option for the encapsulation and osteodifferentiation of BMSCs. (c) 2007 Wiley Periodicals, Inc.