Subcapsular encounter and complement-dependent transport of immune complexes by lymph node B cells
Subcapsular encounter and complement-dependent transport of immune complexes by lymph node B cells
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DOI：
10.1038/ni1494
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发表时间：
2007-09-01
影响因子：
30.5
通讯作者：
Cyster, Jason G.
中科院分区：
文献类型：
--
作者：
Phan, Tri Giang;Grigorova, Irina;Cyster, Jason G.
B细胞-抗原在淋巴组织中相遇的机制尚不完全清楚。目前也不清楚免疫复合体是如何运输到滤泡树突状细胞的。在这里，使用实时双光子显微镜，我们注意到免疫复合体通过淋巴快速传递到淋巴被膜下窦内的巨噬细胞。B细胞通过补体受体依赖的机制从巨噬细胞突起捕获免疫复合体，巨噬细胞突起穿透毛囊并将复合体运输到滤泡树突状细胞。此外，同源B细胞从巨噬细胞突起捕获含有抗原的免疫复合体，并迁移到T区。我们的发现表明，包膜下窦内的巨噬细胞是B细胞与免疫复合物相遇的重要部位，并表明B细胞在毛囊内的迁移不仅有助于免疫复合物的运输，也有助于与同源抗原的相遇。
The mechanism of B cell-antigen encounter in lymphoid tissues is incompletely understood. It is also unclear how immune complexes are transported to follicular dendritic cells. Here, using real-time two-photon microscopy we noted rapid delivery of immune complexes through the lymph to macrophages in the lymph node subcapsular sinus. B cells captured immune complexes by a complement receptor-dependent mechanism from macrophage processes that penetrated the follicle and transported the complexes to follicular dendritic cells. Furthermore, cognate B cells captured antigen-containing immune complexes from macrophage processes and migrated to the T zone. Our findings identify macrophages lining the subcapsular sinus as an important site of B cell encounter with immune complexes and show that intrafollicular B cell migration facilitates the transport of immune complexes as well as encounters with cognate antigen.