Hepatic dendritic cell subsets in the mouse
Hepatic dendritic cell subsets in the mouse
复制标题
DOI：
10.1002/eji.200324336
复制
发表时间：
2004-02-01
影响因子：
5.4
通讯作者：
Reimann, J
中科院分区：
文献类型：
--
作者：
Jomantaite, L;Dikopoulos, N;Reimann, J
小鼠肝脏非实质细胞群中的CD 11 c（+）细胞群包含树突状细胞（DC）、NK细胞、B细胞和T细胞。在免疫活性或免疫缺陷[重组酶激活基因1（RAG 1）（-/-）] C57 BL/6小鼠（T细胞、B细胞和NK细胞严重耗竭）的肝脏CD 11 c（+）DC群中，我们鉴定了B220（+）CD 11 c（int）“浆细胞样”DC亚群和B220（-）CD 11 c（+）DC亚群。后者DC群可细分为主要的未成熟（CD 40（lo）CD 80（lo）CD 86（lo）MHC II类lo）CD 11 c（int）亚群和次要的成熟（CD 40（hi）CD 80（hi）CD 86（hi）MHC II类hi）CD 11 c（hi）亚群。刺激的B220（+）而不是B220（-）DC产生I型干扰素。体内NKT细胞活化在注射后18 h内使肝脏B220（-）DC的数量增加3 - 4倍，并上调其表面活化标记物的表达，同时使B220（+）DC群体收缩。在病毒感染的早期，肝脏B220（+）DC亚群扩增，并且肝脏中的B220（+）和B220（-）DC群体都成熟。在体外，B220（-）而不是B220（+）DC致敏CD 4（+）或CD 8（+）T细胞。不同的标志物特征和功能的表达，以及对激活信号的不同早期反应，因此在从小鼠肝脏新鲜分离的CD 11 c（+）DC群中鉴定出两种不同的B220（+）和B220（-）亚群。
The CD11c(+) cell population in the non-parenchymal cell population of the mouse liver contains dendritic cells (DC), NK cells, B cells and T cells. In the hepatic CD11c(+) DC population from immunocompetent or immuncideficient [recombinase-activating gene-1 (RAG1)(-/-)] C57BL/6 mice (rigorously depleted of T cells, B cells and NK cells), we identified a B220(+) CD11c(int) subset of 'plasmacytoid' DC, and a B220(-)CD11c(+) DC subset. The latter DC population could be subdivided into a major, immature (CD40(lo) CD80(lo) CD86(lo) MHC class IIlo) CD11c(int) subset, and a minor, mature (CD40(hi) CD80(hi) CD86(hi) MHC class IIhi) CD11c(hi) subset. Stimulated B220(+) but not B220(-) DC produced type I interferon. NKT cell activation in vivo increased the number of liver B220(-) DC three- to fourfold within 18 h post-injection, and upregulated their surface expression of activation marker, while it contracted the B220(+) DC population. Early in virus infection, the hepatic B220(+) DC subset expanded, and both, the B220(+) as well as B220(-) DC populations in the liver matured. In vitro, B220(-) but not B220(+) DC primed CD4(+) or CD8(+) T cells. Expression of distinct marker profiles and functions, and distinct early reaction to activation signals hence identify two distinct B220(+) and B220(-) subsets in CD11c(+) DC populations freshly isolated from the mouse liver.