Transcriptomic Analysis Reveals Calcium Regulation of Specific Promoter Motifs in Arabidopsis
Transcriptomic Analysis Reveals Calcium Regulation of Specific Promoter Motifs in Arabidopsis
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DOI：
10.1105/tpc.111.090480
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发表时间：
2011-11-01
期刊：
影响因子：
11.6
通讯作者：
Knight, Marc R.
中科院分区：
文献类型：
--
作者：
Whalley, Helen J.;Sargeant, Alexander W.;Knight, Marc R.
细胞内钙离子浓度（[Ca 2 +] c）的增加通过调节编码赋予耐受性的蛋白质的基因的表达来介导植物对胁迫的响应。一些植物胁迫基因先前已被证明是钙调节的，在一种情况下，一个特定的启动子基序脱落酸响应元件（ABRE）已被发现是由钙调节。利用全基因组微阵列分析，通过测量响应于三种不同特征的钙升高的拟南芥幼苗中的基因表达，对拟南芥钙调节启动子基序的转录组进行了全面调查。这项工作揭示了拟南芥中总共269个基因被[Ca 2 +]（c）上调。生物信息学分析强烈表明，至少有四个启动子基序的[Ca 2 +]（c）-调节在植物中。我们证实了这一发现，在植物中表达嵌合基因结构控制的顺式元件，并通过测试的必要性和足够的钙，其表达。我们的数据表明，C-重复/干旱响应元件，站点II，CAM框（沿着与以前确定的ABRE）启动子基序钙调节。这些启动子元件的第二信使细胞内钙的识别有影响植物信号在响应各种刺激，包括寒冷，干旱和生物胁迫。
Increases in intracellular calcium concentration ([Ca2+] c) mediate plant responses to stress by regulating the expression of genes encoding proteins that confer tolerance. Several plant stress genes have previously been shown to be calcium-regulated, and in one case, a specific promoter motif Abscisic Acid Responsive-Element (ABRE) has been found to be regulated by calcium. A comprehensive survey of the Arabidopsis thaliana transcriptome for calcium-regulated promoter motifs was performed by measuring the expression of genes in Arabidopsis seedlings responding to three calcium elevations of different characteristics, using full genome microarray analysis. This work revealed a total of 269 genes upregulated by [Ca2+](c) in Arabidopsis. Bioinformatic analysis strongly indicated that at least four promoter motifs were [Ca2+](c)-regulated in planta. We confirmed this finding by expressing in plants chimeric gene constructs controlled exclusively by these cis-elements and by testing the necessity and sufficiency of calcium for their expression. Our data reveal that the C-Repeat/Drought-Responsive Element, Site II, and CAM box (along with the previously identified ABRE) promoter motifs are calcium-regulated. The identification of these promoter elements targeted by the second messenger intracellular calcium has implications for plant signaling in response to a variety of stimuli, including cold, drought, and biotic stress.