Enrichment Using an Up-flow Column Reactor and Community Structure of Marine Anammox Bacteria from Coastal Sediment
Enrichment Using an Up-flow Column Reactor and Community Structure of Marine Anammox Bacteria from Coastal Sediment
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DOI：
10.1264/jsme2.me10158
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发表时间：
2011-03-05
影响因子：
2.2
通讯作者：
Ohashi, Akiyoshi
中科院分区：
文献类型：
--
作者：
Kindaichi, Tomonori;Awata, Takanori;Ohashi, Akiyoshi
以添加氮和矿物质的人工海水为营养源，接种广岛湾沿岸表层沉积物，采用上流式反应器，建立了海洋厌氧氨氧化菌的富集培养体系。反应器运行2个月后，观察到NH 4+和NO2-的同时去除，表明厌氧氨氧化反应正在进行。在第594天时，总氮去除率达到2.17 g-N L-1d（-1），而氮负荷为3.33 g-N L-1d（-1）。系统发育分析表明，该反应器中至少存在两种优势种“红背树蛙”。此外，许多未培养的细菌和古菌，包括候选分裂或氨氧化古菌，存在。荧光原位杂交（FISH）显示，厌氧氨氧化细菌占总细菌的85.5 +/- 4.5%，在第393天。我们还设计了两个寡核苷酸探针，具体到每个占主导地位的“”Scalindua物种。使用两种探针的同时FISH分析表明，两种不同的“Escheridatus Scalindua”物种是清楚地可识别的，并在反应堆运行过程中共存，虽然它们的丰度有一些变化。本研究所富集的海洋厌氧氨氧化菌在处理高浓度氨氮和高盐工业废水方面具有潜在的应用价值。
We established an enrichment culture of marine anaerobic ammonium oxidation (anammox) bacteria using an up-flow column reactor fed with artificial sea water supplemented with nitrogen and minerals and inoculated with coastal surface sediment collected from Hiroshima Bay. After 2 months of reactor operation, simultaneous removal of NH4+ and NO2- was observed, suggesting that an anammox reaction was proceeding. A total nitrogen removal rate of 2.17 g-N L-1 day(-1) was attained on day 594 while the nitrogen loading rate was 3.33 g-N L-1 day(-1). Phylogenetic analysis revealed that at least two dominant "Candidatus Scalindua" species were present in this reactor. Moreover, many uncultured bacteria and archaea, including candidate division or ammonia-oxidizing archaea, were present. Fluorescence in situ hybridization (FISH) revealed that anammox bacteria accounted for 85.5 +/- 4.5% of the total bacteria at day 393. We also designed two oligonucleotide probes specific to each dominant "Candidatus Scalindua" species. A simultaneous FISH analysis using both probes showed that two different "Candidatus Scalindua" species were clearly recognizable and coexisted during reactor operation, although there was some variation in their abundance. The marine anammox bacteria enriched in this study have potential applications to the treatment of industrial wastewater containing high levels of ammonium and salt.