Staphylococcus aureus lipoteichoic acid triggers inflammation in the lactating bovine mammary gland
Staphylococcus aureus lipoteichoic acid triggers inflammation in the lactating bovine mammary gland
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DOI：
10.1051/vetres:2008034
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发表时间：
2008-09-01
影响因子：
4.4
通讯作者：
Gilbert, Florence B.
中科院分区：
文献类型：
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作者：
Rainard, Pascal;Fromageau, Angelina;Gilbert, Florence B.
牛乳腺对脂磷壁酸（LTA）的反应，这是革兰氏阳性菌的主要病原体相关的分子模式，通过将纯化的金黄色葡萄球菌LTA输注到乳腺的管腔中来研究。LTA能够在100 μ g/乳区的剂量下诱导临床乳腺炎，并且在10 μ g/乳区诱导亚临床炎症反应。诱导的炎症的特征在于牛奶中的中性粒细胞的迅速和大量流入。证明LTA强烈诱导主要靶向中性粒细胞的趋化因子CXCL 1、CXCL 2、CXCL 3和CXCL 8的分泌。补体衍生的化学引诱物C5a仅在具有最高剂量的LTA（100 μ g）的乳汁中产生。促炎细胞因子IL-1 β在牛奶中被诱导，但如果有TNF-α，则非常少，并且没有IFN-γ。重新评估先前受到沙门氏菌挑战的乳区乳清中的CXCL 8浓度。金黄色葡萄球菌，通过使用针对牛CXCL 8设计的ELISA，显示这种趋化因子在牛奶中被诱导，与先前的报道相矛盾。总体而言，S.金黄色葡萄球菌LTA引起的乳腺炎性反应与S.金黄色乳腺炎纯化的LTA有望成为研究乳腺对S.金黄色的
The response of the bovine mammary gland to lipoteichoic acid (LTA), which is a major pathogen-associated molecular pattern of Gram-positive bacteria, was investigated by infusing purified Staphylococcus aureus LTA in the lumen of the gland. LTA was able to induce clinical mastitis at the dose of 100 mu g/quarter, and a subclinical inflammatory response at 10 mu g/quarter. The induced inflammation was characterized by a prompt and massive influx of neutrophils in milk. LTA proved to induce strongly the secretion of the chemokines CXCL1, CXCL2, CXCL3 and CXCL8, which target mainly neutrophils. The complement-derived chemoattractant C5a was generated in milk only with the highest dose of LTA (100 mu g). The pro-inflammatory cytokine IL-1 beta was induced in milk, but there was very little if any TNF-alpha and no IFN-gamma. The re-assessment of CXCL8 concentrations in milk whey of quarters previously challenged with S. aureus, by using an ELISA designed for bovine CXCL8, showed that this chemokine was induced in milk, contradicting previous reports. Overall, S. aureus LTA elicited mammary inflammatory responses that shared several attributes with S. aureus mastitis. Purified LTA looks promising as a convenient tool to investigate the inflammatory and immune responses of the mammary gland to S. aureus.