The immunomodulatory effects of the herbicide propanil on murine macrophage interleukin-6 and tumor necrosis factor-alpha production
The immunomodulatory effects of the herbicide propanil on murine macrophage interleukin-6 and tumor necrosis factor-alpha production
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DOI：
10.1006/taap.1997.8179
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发表时间：
1997-07-01
影响因子：
3.8
通讯作者：
Barnett, JB
中科院分区：
文献类型：
--
作者：
Xie, YC;Schafer, R;Barnett, JB
腹膜内)暴露于丙胺(3，4-二氯丙酰苯胺)之前已被证明会影响巨噬细胞的细胞毒性。在这项研究中，我们比较了丙醇在体内不同途径给药后对硫代乙酸酯诱导的巨噬细胞产生细胞因子的免疫毒性作用。C57B1/6小鼠ip溶媒或200 mg/kg丙醇，或灌胃溶媒、40或400 mg/kg丙醇。3天或7天后，测定内毒素刺激后巨噬细胞产生白介素6(IL-6)和肿瘤坏死因子-α(TNF-α)的量。口服和ip丙醇均可导致内毒素刺激的巨噬细胞产生的IL-6和TNF-α减少60%-70%，这取决于给药的途径、暴露后的时间和剂量。口服丙胺也会导致动物的脾肿大和胸腺萎缩，其方式与通过ip途径暴露的动物大致相同。在体外，暴露于丙胺也显著减少巨噬细胞细胞因子的产生。用硫代乙醇酸诱导的正常小鼠巨噬细胞在0、10或20 mU丙醇+脂多糖的持续存在下培养。这种暴露以浓度依赖的方式导致这些巨噬细胞产生的IL-6和肿瘤坏死因子蛋白显著减少。Northern印迹分析表明，这些细胞因子的信息水平与丙醇处理的巨噬细胞的蛋白水平降低的百分比大致相同，这表明丙醇可能是一个转录或转录前靶点(S)。(C)1997年学术出版社。
Intraperitoneal tip) exposure to propanil (3,4-dichloropropionanilide) has previously been shown to affect macrophage cytotoxicity. In this study, we compared the immunotoxic effects of propanil, after different routes of in vivo administration, on cytokine production by thioglycollate-elicited peritoneal macrophages. C57B1/6 mice were treated with either vehicle or 200 mg/kg propanil ip, or with vehicle, 40, or 400 mg/kg propanil orally. Three or 7 days later, ex vivo production of interleukin-6 (IL-6) and tumor necrosis factor-alpha (TNF-alpha) by macrophages after lipopolysaccharide OBS) stimulation was determined. Both oral and ip propanil exposure resulted in up to a 60-70% reduction in IL-6 and TNF-alpha production by the LPS-stimulated macrophages, depending on the route, postexposure time, and dose of propanil administered. Oral exposure to propanil also caused splenomegaly and thymic atrophy in animals in much the same manner as animals exposed via the ip route. In vitro exposure to propanil also significantly reduced macrophage cytokine production. Thioglycollate-elicited macrophages from normal mice were cultured in the continuous presence of 0, 10, or 20 mu M propanil plus LPS. This exposure caused a significant reduction in IL-6 and TNF protein production by these macrophages in a concentration-dependent manner. Northern blot analysis demonstrated that the message levels of these cytokines were reduced by approximately the same percentage as the protein levels in propanil-treated macrophages, indicating a possible transcriptional or pretranscriptional target(s) for propanil. (C) 1997 Academic Press.