PARATHYROID HORMONE-STIMULATED DEVELOPMENT OF OSTEOCLASTS IN CULTURES OF CELLS FROM NEONATAL MURINE CALVARIA
PARATHYROID HORMONE-STIMULATED DEVELOPMENT OF OSTEOCLASTS IN CULTURES OF CELLS FROM NEONATAL MURINE CALVARIA
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DOI：
10.1016/8756-3282(86)90149-3
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发表时间：
1986-01-01
期刊：
影响因子：
4.1
通讯作者：
JILKA, RL
中科院分区：
文献类型：
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作者：
JILKA, RL
通过连续酶消化法从新生鼠颅骨制备的细胞中存在破骨细胞或破前骨细胞的证据。新鲜分离的细胞在消化过程后期释放的再吸收活的和失活的颅骨骨基质，以响应PTH，伴随着破骨细胞的发展。在培养1至2天后，这些细胞的光学和扫描电子显微镜显示存在圆形细胞（10至15 μ m）。直径），除了较大的成骨细胞外，表面微绒毛最少。少数细胞含有抗酒石酸盐酸性磷酸酶（TRANS 3）。如果最初以汇合密度接种，在随后的培养过程中会产生更多的TRA-BRA阳性细胞，并且这些细胞在用甲状旁腺激素（PTH）处理后保留了再吸收颅骨骨基质的能力。这些培养的细胞对PTH有反应，TRA-PTH分泌到培养基中增加，TRA-PTH阳性细胞数量增加。这些是20至50 μ。直径，有时是多核的，有些散布100 - 200 μ m。长的对摄取中性红的活细胞的观察表明，在用降钙素（CT）处理后，一些但不是所有标记细胞的表面丝状伪足在30分钟内缩回。如果细胞最初以低密度接种并在暴露于激素之前生长至汇合，则会发生对PTH的再吸收反应的丧失以及PTH刺激TRAPase阳性细胞的发育。这与大多数10至15 μ C的损失相关。直径圆形细胞。这些观察结果表明，破骨细胞前体存在于这些小细胞中，这些小细胞可以在用PTH治疗后产生破骨细胞。
Evidence was sought for the presence of osteoclasts or preosteoclasts in cells prepared from neonatal murine calvaria by sequential enzymatic digestion. Freshly isolated cells released late in the digestion process resorbed both living and devitalized calvarial bone matrix in response to PTH, accompanied by the development of osteoclasts. Light and scanning electron microscopy of these cells after 1 to 2 days in culture revealed the presence of round cells (10 to 15 .mu. in diameter) with minimal surface microvilli in addition to the larger osteoblastic cells. Few cells contained tartrate-resistant acid phosphatase (TRAPase). If initially seeded at confluent density, more cells positive for TRA-Pase developed during subsequent culture, and these cells retained the ability to resorb calvarial bone matrix upon treatment with parathyroid hormone (PTH). These cultured cells responded to PTH with increased secretion of TRA-Pase into the medium and increased numbers of TRAPase positive cells. These were 20 to 50 .mu. in diameter, sometimes multinucleated, and some were spread 100 to 200 .mu. in length. Observations of living cells that took up neutral red showed that, upon treatment with calcitonin (CT), surface filopodia of some but not all of the labeled cells retracted within 30 minutes. Loss of resorptive response to PTH, as well as PTH-stimulated development of TRAPase-positive cells, occurred if the cells were initially seeded at low density and grown to confluence before exposure to hormone. This correlated with the loss of most of the 10 to 15 .mu. diameter round cells. These observations suggest that preosteoclasts are present among these small cells that can give rise to osteoclasts upon treatment with PTH.