Descemet membrane air‐bubble separation in donor corneas
Descemet membrane air‐bubble separation in donor corneas
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DOI：
10.1016/j.jcrs.2010.10.013
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发表时间：
2010-12
影响因子：
2.8
通讯作者：
D. Venzano;P. Pagani;Nadia Randazzo;F. Cabiddu;C. Traverso
中科院分区：
文献类型：
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作者：
D. Venzano;P. Pagani;Nadia Randazzo;F. Cabiddu;C. Traverso
我们描述了一种技术，以获得后弹力层内皮光盘从捐助者。为了分离德斯密膜，将气泡引入安装在人工室上的人供体角膜的深层基质中。A组（n= 5），泡沫保持膨胀。B组（n= 4），膜脱落后立即放气。C组（n= 7），在泡沫破裂后，将后弹力层-内皮盘环钻并与基质分离。所有组织均在4 ℃下保存.在89%的组织中实现了后弹力层脱离。48小时后，3组的平均内皮损失分别为83%±10%（SD）、15%± 11%和3%±3%。用这种技术，后弹力层内皮圆盘获得没有显着改变的内皮层。
We describe a technique to obtain Descemet–endothelium disks from donors. To detach Descemet membrane, an air bubble was introduced in the deep stroma of human donor corneas mounted on an artificial chamber. In Group A (n= 5), the bubble was left inflated. In Group B (n= 4), the bubble was deflated immediately after the membrane was detached. In Group C (n= 7), the Descemet–endothelium disk was trephined and separated from the stroma after the bubble was deflated. All tissues were stored at 4 C. Descemet detachment was achieved in 89% of the tissues. After 48 hours, the mean endothelial loss was 83%±10%(SD), 15%±11%, and 3%±3% in the 3 groups, respectively. With this technique, Descemet–endothelium disks were obtained without significant alterations in the endothelial layer.