Metabolism of Escherichia coli injured by copper.
Metabolism of Escherichia coli injured by copper.
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DOI：
10.1139/m87-010
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发表时间：
1987
影响因子：
2.8
通讯作者：
M. Domek;J. Robbins;M. Anderson;G. McFeters
中科院分区：
文献类型：
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作者：
M. Domek;J. Robbins;M. Anderson;G. McFeters
在模拟饮用水环境的碳酸盐缓冲液中，铜对大肠杆菌的损伤使其氧利用率降低。Oxygraph测量结果显示，铜损伤的细菌的氧利用率是控制细胞的不到25%。呼吸测定实验在较长时间内测量速率，并显示出类似的趋势。采用核磁共振波谱（13 C nmr）和气相色谱法来鉴定健康和受伤的E.杆菌缺氧条件下受损细胞对葡萄糖的利用率为健康细胞的64%。乳酸和乙醇积累率分别为对照的88%和50%。氧合培养物的13 C NMR研究揭示了乙酸盐和谷氨酰胺积累的差异。损伤细胞对葡萄糖和琥珀酸的有氧利用率分别为对照组的87%和21%。其他研究显示，受损细胞用各种碳水化合物底物还原2-（对碘苯基）-3-（对硝基苯基）-5-苯基氯化四唑（INT）的能力降低。当NADH被用作底物时，受损细胞比健康细胞减少了更大量的INT。代谢终产物的比较表明，与健康细胞相比，受损细胞的碳流也有相当大的差异。
Escherichia coli injured by copper in carbonate buffer simulating the drinking water environment showed decreased oxygen utilization. Oxygraph measurements revealed that copper-injured bacteria had a rate of oxygen utilization that was less than 25% of that of control cells. Respirometry experiments measured rates over a longer period of time and showed similar trends. Nuclear magnetic resonance spectroscopy (13C nmr) and gas chromatography were used to identify differences in metabolism between healthy and injured populations of E. coli. The rate of glucose utilization by injured cells under anaerobic conditions was 64% of that of healthy cells. The rates of lactate and ethanol accumulation were 88 and 50% of the control, respectively. The 13C nmr studies of oxygenated cultures revealed differences in the accumulation of acetate and glutamine. Aerobic utilization of glucose and succinate by injured cells were 87 and 21% of the rate of the controls, respectively. Additional studies revealed injured cells had a decreased ability to reduce 2-(p-iodophenyl)-3-(p-nitrophenyl)-5-phenyltetrazolium chloride (INT) with a variety of carbohydrate substrates. Injured cells reduced greater quantities of INT than healthy cells when NADH was used as a substrate. A comparison of metabolic end products suggested that injured cells also had considerable differences in carbon flow compared with healthy cells.