Biological markers in nasal secretions
Biological markers in nasal secretions
复制标题
DOI：
10.1183/09031936.03.00072003
复制
发表时间：
2003-04-01
影响因子：
24.3
通讯作者：
Bachem, M
中科院分区：
文献类型：
--
作者：
Riechelmann, H;Deutschle, T;Bachem, M
鼻分泌物中的生物标记物为鼻病理生理学提供了有价值的信息。然而，已发表的鼻液中生物标志物浓度的数据是非常不一致的，由于不同的采样技术，尚未进行系统的评估。通过使用：1）α（2）-巨球蛋白作为血浆污染的标志物; 2）乳铁蛋白作为腺体分泌的标志物; 3）乳酸脱氢酶作为组织损伤的标志物;和4）白细胞介素（IL）-1 β、IL-8、肿瘤坏死因子-α和嗜酸性粒细胞阳离子蛋白和类胰蛋白酶作为组织炎症的指标来检测蛋白质。共采用了四种不同的采样方法，包括鼻腔灌洗（NL）和一种新的聚氨酯泡沫采样器技术（PFST）。NL中的分析物浓度比PFST获得的样本低约10倍。由于NL期间不可预测的稀释，各种分析物低于所采用的高灵敏度测定的检测限。使用PFST时，很少出现低于检测限的浓度。这些样本在血浆污染、腺体分泌物或组织损伤方面没有显著差异。鼻分泌物中报告的分析物浓度的相当大的变异性主要来自不同的采样技术。为了收集鼻分泌物，认为采样器比鼻灌洗技术优越上级。
Biological markers in nasal secretions provide valuable information on nasal pathophysiology. However, published data on biomarker concentrations in nasal fluids are remarkably inconsistent, and the bias due to different sampling techniques, has not yet been systematically evaluated.Concentrations of various protein were repeatedly determined in nasal secretions of 16 healthy volunteers. The proteins were detected by using: 1) alpha(2)-macroglobulin as a marker for plasma contamination; 2) lactoferrin as a marker for glandular secretion; 3) lactate dehydrogenase as a marker for tissue injury; and 4) interleukin (IL)-1beta, IL-8, tumour necrosis factor-alpha, and eosinophil cationic protein and tryptase as indicators for tissue inflammation. A total of four different sampling methods, including nasal lavage (NL) and a new polyurethane foam sampler technique (PFST) were employed.Analyte concentrations in NL were approximately 10-times lower than in specimens obtained by PFST. Due to the unpredictable dilution during NL, various analytes were below the detection limit of the high sensitivity assays employed. With PFST, concentrations below the detection limit rarely occurred. The specimens did not significantly differ regarding plasma contamination, glandular secretion or tissue injury.The considerable variability of reported analyte concentrations in nasal secretions mainly results from different sampling techniques. To collect nasal secretions, samplers are considered superior to nasal lavage techniques.