Electrically silent potassium channel subunits from human lens epithelium.
Electrically silent potassium channel subunits from human lens epithelium.
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来自人类晶状体上皮的电沉默钾通道亚基。
DOI：
10.1152/ajpcell.1999.277.3.c412
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发表时间：
1999
期刊：
影响因子：
--
通讯作者：
Rae,JL
中科院分区：
文献类型：
--
作者：
Shepard,AR;Rae,JL
我们描述了电沉默延迟校正样K+通道亚家族的第一个人类成员hKv9.1和hKv9.3的克隆和特征。它们对电活性亚家族成员hKv2.1的调节作用也被量化。从人晶状体上皮cDNA文库中分离出hKv9 K+通道，但在大量人体组织中发现hKv9.1 mRNA和hKv9.3 mRNA与hKv2.1 mRNA共存。hKv9.1基因由至少5个外显子组成，其中至少两个外显子位于5 ' -非翻译区（UTR）。相比之下，hKv9.3基因在整个编码区，3 ' -UTR和所有分析的5 ' -UTR中都是无内含子的。辐射杂交定位将hKv9.1基因定位于20q12， hKv9.3基因定位于2p24。当与hKv2共表达时，每个电沉默亚基。l，与单独表达的hKv2.1相比，减慢了失活和失活。此外，每一种都会导致单通道电导的增加。
We describe the cloning and characterization of the first human members, hKv9.1 and hKv9.3, of the electrically silent delayed-rectifying-like K+channel subfamily. Their modulatory effects on the electrically active subfamily member hKv2.1 are also quantified. The hKv9 K+channels were isolated from a human lens epithelium cDNA library, but both hKv9.1 mRNA and hKv9.3 mRNA were found to coexist with the mRNA for hKv2.1 in a large number of human tissues. The hKv9.1 gene is composed of a minimum of five exons, with at least two alternatively spliced exons in the 5′-untranslated region (UTR). In contrast, the hKv9.3 gene is intronless across the coding region, 3′-UTR, and all of the analyzed 5′-UTR. Radiation hybrid mapping localized the hKv9.1 gene to 20q12 and the hKv9.3 gene to 2p24. Each electrically silent subunit, when coexpressed with hKv2.l, slows deactivation and inactivation compared with hKv2.1 expressed alone. In addition, each results in an increment in the single channel conductance.