SELECTIVE-INHIBITION OF COLLAGEN ACCUMULATION BY N-(3,4-DIMETHOXYCINNAMOYL)ANTHRANILIC ACID (N-5') IN GRANULATION-TISSUE
SELECTIVE-INHIBITION OF COLLAGEN ACCUMULATION BY N-(3,4-DIMETHOXYCINNAMOYL)ANTHRANILIC ACID (N-5') IN GRANULATION-TISSUE
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DOI：
10.1016/0006-2952(87)90353-4
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发表时间：
1987-02-15
影响因子：
5.8
通讯作者：
NAITO, J
中科院分区：
文献类型：
--
作者：
ISAJI, M;NAKAJOH, M;NAITO, J
研究了局部应用 N-5''（肥大细胞释放化学介质的抑制剂）对角叉菜胶气囊炎症的影响。 N-5''（100mg/kg）处理显着减少了肉芽组织的形成、渗出物的积累和浸润细胞的数量。通过N-5''处理，肉芽组织中的胶原蛋白含量呈剂量依赖性降低，但不影响非胶原蛋白和DNA含量。 N-5''剂量为100 mg/kg时，组织中脯氨酰羟化酶活性显着降低。肉芽组织中胶原蛋白积累的选择性抑制是由于体内胶原蛋白生物合成的减少所致。 N-5''不直接抑制二倍体成纤维细胞的胶原合成，但抑制培养物中成纤维细胞的增殖。这些结果表明，N-5''在炎症部位抑制胶原蛋白积累的机制之一可能涉及抑制成纤维细胞增殖。
The effect of topically applied N-5'', an inhibitor of chemical mediator release from mast cells, on the carrageenin-air-pouch inflammation was studied. The formation of granulation tissue, the accumulation of exudate and the number of infiltrating cells were significantly reduced by the treatment with N-5'' (100 mg/kg). The collagen content in granulation tissue was dose-dependently reduced without affecting the noncollagen protein and DNA content by treatment with N-5''. At a dose of 100 mg/kg of N-5'', prolyl hydroxylase activity in the tissue was significantly decreased. The selective inhibition of collagen accumulation in granulation tissue resulted from reduction of collagen biosynthesis in vivo. N-5'' did not directly inhibit collagen synthesis by diploid fibroblasts, but inhibited fibroblast proliferation in culture. Such results indicate that one of the inhibitory mechanisms of collagen accumulation by N-5'' in inflamed sites may involve the inhibition of fibroblast proliferation.