Tetrodotoxin-resistant impulses in single nociceptor nerve terminals in guinea-pig cornea
Tetrodotoxin-resistant impulses in single nociceptor nerve terminals in guinea-pig cornea
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DOI：
10.1111/j.1469-7793.1998.211bf.x
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发表时间：
1998-10-01
影响因子：
5.5
通讯作者：
Belmonte, C
中科院分区：
文献类型：
--
作者：
Brock, JA;McLachlan, EM;Belmonte, C
1. 细胞外记录技术已被用于研究离体豚鼠角膜单个感觉神经末梢的神经冲动。神经冲动是自发产生的，由睫状肌神经的电刺激引起。神经冲动被确定为起源于多模态受体、机械受体或“冷”受体。这三种类型都被认为是伤害感受器。河豚毒素（TTX, 1 μ M）阻断睫状神经电刺激引起的神经冲动。然而，当TTX存在于所有三种类型的受体中时，持续和/或自然诱发的神经冲动持续存在。利多卡因阻断了所有的脑电活动。因此，抗ttx钠通道在产生向大脑发出疼痛信号的动作电位中起着重要作用。
1. Extracellular recording techniques have been used to study nerve impulses in single sensory nerve terminals in guinea-pig cornea isolated in vitro.2. Nerve impulses occurred spontaneously and were evoked by electrical stimulation of the ciliary nerves.3. The nerve impulses were identified as originating in polymodal receptors, mechanoreceptors or 'cold' receptors. All three types are believed to be nociceptors.4. Tetrodotoxin (TTX, 1 mu M) blocked nerve impulses evoked by electrical stimulation of the ciliary nerves. However, ongoing and/or naturally evoked nerve impulses persisted in the presence of TTX in all three types of receptors. Lignocaine (lidocaine; I mM) blocked all electrical activity.5. TTX-resistant sodium channels therefore play a major role in generating the action potentials that signal pain to the brain.