A scanning electron microscopic study of peripheral nerve degeneration and regeneration
A scanning electron microscopic study of peripheral nerve degeneration and regeneration
复制标题
周围神经变性与再生的扫描电镜研究
DOI：
10.1016/0306-4522(78)90144-6
复制
发表时间：
1978
期刊：
影响因子：
3.3
通讯作者：
F. Roisen
中科院分区：
文献类型：
--
作者：
M. Gershenbaum;F. Roisen
暴露雄性Holtzman大鼠的左侧坐骨神经，并用止血钳在臀下神经与胫神经和腓总神经分叉之间的中点处挤压3 s。正常的有髓轴突在扫描电镜下表现为相对光滑的圆柱形结构，其长度上有相互交织的胶原纤维。朗氏结沿有髓纤维呈沿着缩窄。压碎后，神经纤维肿胀，在结和结间区域出现中断，导致珠状形态。随着退变过程的进行，髓鞘碎裂成许多球状体。损伤后60天，在神经内发现一些髓鞘碎片的残留物。在损伤后约15天，在结缔组织纤维内观察到一些再生的有髓轴突，并且在压碎区域远端的髓鞘碎片内观察到一些再生的有髓轴突。到第30天，神经在外观上相对正常。扫描电子显微照片提供了神经变性和再生过程中发生的动态过程的三维图像。这些发现与以前的研究相关，可以作为未来扫描电子显微镜研究的基础。
The left sciatic nerves of male Holtzman rats were exposed and crushed with a hemostat for 3 s at a point midway between the inferior gluteal nerve and the bifurcation into the tibial and common peroneal nerves. Normal myelinated axons appear in the scanning electron microscope as relatively smooth cylindrical structures with interweaving strands of collagen fibers coursing over their length. Nodes of Ranvier are seen as constrictions along the myelinated fibers. After crushing, the nerve fibers swell, and interruptions appear in the nodal and internodal regions causing a beaded morphology. The myelin sheaths fragment into numerous spherical bodies as the degenerative process progresses. Some remnants of myelin debris are found within the nerves 60 days following the lesion A few regenerating myelinated axons are observed within the connective tissue fibers and myelin debris distal to the crushed regions approx 15 days after placement of the lesion. By day 30 the nerves are relatively normal in appearance. The scanning electron micrographs provide a three-dimensional picture of the dynamic processes which occur during nerve degeneration and regeneration. These findings are correlated with previous studies and can serve as a basis for future scanning electron microscopic studies in this area of research.