MiR-1271 Inhibits Ovarian Cancer Growth by Targeting Cyclin G1.
MiR-1271 Inhibits Ovarian Cancer Growth by Targeting Cyclin G1.
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DOI：
10.12659/msm.895562
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发表时间：
2015-10-19
期刊：
影响因子：
--
通讯作者：
Wang X
中科院分区：
文献类型：
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作者：
Liu X;Ma L;Rao Q;Mao Y;Xin Y;Xu H;Li C;Wang X
卵巢癌是女性生殖系统中最致命的妇科恶性肿瘤。miRNA功能障碍与卵巢癌的发生有关。采用qRT-PCR检测卵巢癌组织和细胞中miR-1271水平。miRNA-mimic转染使细胞内miR-1271过表达，mirna -反义寡核苷酸（ASO）转染使细胞内miR-1271表达降低。MTT法检测细胞增殖。目标基因通过生物信息学算法预测，并通过双荧光素酶报告基因试验确认。Western blotting检测蛋白水平。卵巢癌组织和细胞系中miR-1271表达水平较低。卵巢癌组织中miR-1271的低水平与患者生存率低相关，并且miR-1271的过表达抑制卵巢癌细胞的增殖。cyclin G1的3 ' UTR （CCNG1）被miR-1271靶向。卵巢癌组织中低水平的miR-1271促进癌细胞生长。MiR-1271可能是卵巢癌预后的新预测因子。MiR-1271通过靶向CCNG1发挥作用。
Ovarian cancer is the most lethal gynecological malignant cancer in the female genital system. The dysfunction of miRNA contributes to ovarian cancer development. The miR-1271 level in ovarian cancer tissues and cells was assayed by qRT-PCR. The miR-1271 expression in cells was overexpressed by miRNA-mimic transfection and reduced by miRNA-antisense-oligonucleotide (ASO) transfection. Cell proliferation was analyzed by an MTT assay. The targeted genes were predicted by a bioinformatics algorithm and confirmed by a dual luciferase reporter assay. The protein level was assayed by Western blotting. The ovarian cancer tissue and cell lines showed low levels of miR-1271. Low levels of miR-1271 in ovarian cancer tissues were correlated with a low rate of patient survival, and the overexpression of miR-1271 inhibited the proliferation of ovarian cancer cells. The 3′ UTR of cyclin G1 (CCNG1) was targeted by miR-1271. Low levels of miR-1271 in ovarian cancer tissues promoted cancer cell growth. MiR-1271 may be a new predictor of prognosis in ovarian cancer. MiR-1271 exerted its role by targeting CCNG1.