Molecular characterization of erythromycin and tetracycline-resistant Enterococcus faecalis isolated from retail chicken meats
Molecular characterization of erythromycin and tetracycline-resistant Enterococcus faecalis isolated from retail chicken meats
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DOI：
10.3382/ps/pey477
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发表时间：
2019-02-01
期刊：
影响因子：
4.4
通讯作者：
Lee, Young Ju
中科院分区：
文献类型：
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作者：
Kim, Yeong Bin;Seo, Kwang Won;Lee, Young Ju
粪肠球菌（Enterococcus faecalis）是一种普遍存在于人类和动物体内的肠道细菌，容易获得抗微生物药物耐药性，这使其能够发挥抗微生物药物耐药性指标的作用。本研究的目的是表征零售鸡肉中分离的红霉素和四环素耐药粪肠球菌。从7个肉鸡综合养殖场分离的335株粪肠球菌中，有149株对红霉素和四环素同时耐药，其中50%以上的菌株对多药耐药。最常见的耐药基因为ermB（96.0%， 143株）和tet(M)（95.3%， 142株）和tet(L)（89.3%， 133株），与大环内酯类药物耐药相关。此外，140株（93.9%）同时具有ermB、tet(L)和/或tet(M)基因。检测到8株含有tn916 /1545样转座子，同时携带ermB和tet(M)基因。最常见的毒力基因为gelE（142株，95.3%）、ace（137株，91.9%）和efaA（120株，80.5%）。此外，5株粪肠杆菌成功地将抗菌和毒力基因转移到粪肠杆菌FA2-2中。因此，耐药粪肠杆菌分离株及其相应的基因和转座子等可移动遗传元件可能通过韩国肉鸡操作系统在全国范围内传播。
Enterococcus faecalis is a ubiquitous intestinal bacterium in human and animals that can easily acquire antimicrobial resistance, which allows it to play the role of an antimicrobial resistance indicator. The objectives of this study were to characterize erythromycin and tetracycline-resistant E. faecalis isolated from retail chicken meats. A total of 149 among 335 E. faecalis isolates from 7 integrated broiler operations showed the simultaneous resistance to erythromycin and tetracycline, and more than 50% among 149 isolates showed multidrug resistance. The most common resistance genes were ermB (96.0%, 143 isolates) related with macrolides resistance, and tet(M) (95.3%, 142 isolates) and tet(L) (89.3%, 133 isolates) related with tetracycline resistance. Furthermore, 140 (93.9%) isolates simultaneously possessed ermB, and tet(L) and/or tet(M) genes. Eight isolates with transposon of the Tn916/1545-like were detected, which also carried ermB and tet(M) genes. The most prevalent of virulence genes were gelE (142 isolates, 95.3%), ace (137 isolates, 91.9%), and efaA (120 isolates, 80.5%). Also, 5 E. faecalis isolates successfully transferred antimicrobial and virulence genes to E. faecalis FA2-2. Therefore, the antimicrobial-resistant E. faecalis isolates as well as their corresponding genes and mobile genetic elements, such as transposons may be disseminated nationwide by broiler operation system in Korea.