KARYOLOGY AND CYTOPHOTOMETRIC ESTIMATION OF INTERSPECIFIC AND INTRASPECIFIC NUCLEAR-DNA VARIATION IN 4 SPECIES OF PORPHYRA (RHODOPHYTA)
KARYOLOGY AND CYTOPHOTOMETRIC ESTIMATION OF INTERSPECIFIC AND INTRASPECIFIC NUCLEAR-DNA VARIATION IN 4 SPECIES OF PORPHYRA (RHODOPHYTA)
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DOI：
10.2216/i0031-8884-30-5-458.1
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发表时间：
1991-09-01
期刊：
影响因子：
1.6
通讯作者：
LEMUS, AJ
中科院分区：
文献类型：
--
作者：
KAPRAUN, DF;HINSON, TK;LEMUS, AJ
对西大西洋温带和热带地区的4种紫菜（Porphyra）的细胞遗传学研究表明，白纹紫菜（P. leucosticta Thuret in Le Jolis）和螺旋紫菜（P. spiralis Oliveira Filho et科尔var. Oliveira Filho et科尔包括具有三条和四条染色体的群体。 使用DNA定位荧光染料氢乙啶的显微分光光度法来估计核基因组大小。 将被子植物金鱼草（Antirrhinum majus Linaeus）和拟南芥（Arabidopsis thaliana（Linnaeus）Heynhold）以及绿色海藻白刚毛藻（Cladophora albida（哈德逊）Kutzing）的相对荧光（I（f））值相对于它们已知的DNA含量作图，以得到标准线。 平均核DNA（I（f））值的紫菜spermatia外推，以获得其基因组大小的估计。 结果表明，在所检查的分离株中，相对较小的值范围较窄（0.93-1.18 pg/2 C基因组）。 白纹伊蚊和旋毛虫的三染色体和四染色体种群的核基因组大小相似。Escherifolia表明，融合/易位过程，而不是染色体丢失/获得伴随着这些实体的物种形成。
A cytogenetic investigation of four Porphyra species (Rhodophyta) from the temperate and tropical western Atlantic indicates that P. leucosticta Thuret in Le Jolis and P. spiralis Oliveira Filho et Coll var. amplifolia Oliveira Filho et Coll include populations with three and four chromosomes. Microspectrophotometry with the DNA-localizing fluorochrome hydroethidine was used to estimate nuclear genome sizes. Relative fluorescence (I(f)) values for the angiosperms Antirrhinum majus Linaeus and Arabidopsis thaliana (Linnaeus) Heynhold, and the green seaweed Cladophora albida (Hudson) Kutzing were plotted against their known DNA contents in order to derive a standard line. Mean nuclear DNA (I(f)) values for Porphyra spermatia were extrapolated to obtain estimates of their genome sizes. The results indicate a narrow range of relatively small values (0.93-1.18 pg/2 C genome) in the isolates that were examined. Similarities in nuclear genome size for both three- and four-chromosome populations of P. leucosticta and P. spiralis var. amplifolia suggest that fusion/translocation processes rather than chromosome loss/gain accompanied speciation in these entities.