Catalytic studies of lipase on FAME production from waste cooking palm oil in a tert-butanol system
Catalytic studies of lipase on FAME production from waste cooking palm oil in a tert-butanol system
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DOI：
10.1016/j.procbio.2008.08.010
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发表时间：
2008-12-01
影响因子：
4.4
通讯作者：
Kamaruddin, Azlina Harun
中科院分区：
文献类型：
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作者：
Halim, Siti Fatimah Abdul;Kamaruddin, Azlina Harun
以食用废棕榈油为原料，采用脂肪酶催化反应制备脂肪酸甲酯(FAME)。对几种商品脂肪酶和不同有机溶剂的催化活性进行了筛选。结果表明，Novozyme 435对WCPO与甲醇的酯交换反应有较好的催化作用。以叔丁醇为反应介质，消除了甲醇过量和甘油副产物对反应的负面影响。在间歇反应体系中考察了醇油摩尔比、脂肪酶用量、搅拌速度等因素对反应的影响。在最佳反应条件下，当醇油摩尔比为4：1，Novozyme435基础油质量分数为4%，转数为200rpm，反应时间为12h时，WCPO的酯交换产率可达88%。研究了叔丁醇体系中脂肪酶催化WCPO的酯交换反应动力学。建立了仅受甲醇抑制的乒乓铋模型，拟合出初始速率数据，并用非线性回归分析求出了动力学参数。(C)2008爱思唯尔有限公司。保留所有权利。
In this present work, fatty acid methyl ester (FAME) was produced from waste cooking palm oil (WCPO) by lipase-catalyzed reaction. The catalytic activities of several commercial lipases and different organic solvent were screened. Novozyme 435 was found to be more effective in catalyzing the transesterification of WCPO with methanol. tert-Butanol was used as the reaction medium, which eliminated both negative effect caused by excessive methanol and glycerol as the byproduct. Several variables such as effect of methanol/oil molar ratio, effect of lipase quantity and effect of agitation speed (rpm) were examined in a batch system. Transesterification of WCPO can reach up to 88% FAME yield under optimum condition (methanol/oil molar ratio 4:1, 4% Novozyme 435 based oil oil weight, 200 rpm and 12 h reaction time). Kinetics of lipase-catalyzed transesterification of WCPO in tert-butanol system was also investigated. A model based on Ping Pong Bi Bi with only inhibition by methanol was found to fit the initial rate data and the kinetics parameters were evaluated by non-linear regression analysis. (c) 2008 Elsevier Ltd. All rights reserved.