A valine421 to methionine mutation in IS6 of the hscp voltage-gated sodium channel associated with pyrethroid resistance in Heliothis virescens F.
A valine421 to methionine mutation in IS6 of the hscp voltage-gated sodium channel associated with pyrethroid resistance in Heliothis virescens F.
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DOI：
10.1006/bbrc.1997.7511
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发表时间：
1997-10-29
影响因子：
3.1
通讯作者：
Feyereisen, R
中科院分区：
文献类型：
--
作者：
Park, Y;Taylor, MFJ;Feyereisen, R
编码电压门控钠通道的基因座上的多个突变已被预测为昆虫对拟除虫菊酯的抗性。在此之前，我们报道了一个与拟除虫菊酯抗性相关的突变，Leu1029到His，在绿色日光夜蛾HSCP基因座的结构域II跨膜片段S6(IIS6)上。对HSCP基因座上该突变的额外抗性单倍型5‘的序列分析发现，在IS6(V421M，睡果蝇)的氨基酸位置上，G到A的转变导致了Val到Met的突变。V421M突变仅在一个独特的抗性单倍型中发现，而在携带L1029H突变的两个易感单倍型和一个独特的抗性单倍型中未发现。讨论了这一发现对拟除虫菊酯抗性进化和机制的影响。(C)1997年学术出版社。
Multiple mutations in a locus encoding a voltage-gated sodium channel have been predicted for pyrethroid resistance in insects. Previously we reported a mutation associated with pyrethroid resistance, Leu1029 to His, in domain II transmembrane segment S6 (IIS6) of the Heliothis virescens F. sodium channel (para homologue) hscp locus. Sequence analysis of additional resistance haplotypes 5' to this mutation in the hscp locus has uncovered a G to A transition leading to a Val to Met mutation at amino acid position 421 in IS6 (V421M, slumbering from Drosophila para). The V421M mutation is found only in a unique resistant haplotype, hut not in two susceptible and a distinct resistant haplotype carrying the L1029H mutation. Implications of this finding in the evolution and mechanisms of pyrethroid resistance are discussed. (C) 1997 Academic Press.