Dexamethasone Inhibits Formation of Osteoclast-like Cells in Bone-marrow Cultures
Dexamethasone Inhibits Formation of Osteoclast-like Cells in Bone-marrow Cultures
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地塞米松抑制骨髓培养物中破骨细胞样细胞的形成
DOI：
10.1177/00220345860650070201
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发表时间：
1986
影响因子：
7.6
通讯作者：
J. Heersche
中科院分区：
文献类型：
--
作者：
M. Pharoah;J. Heersche
我们的观察结果证实了先前报道的观察，即多核细胞在猫骨髓培养物中在两周内发育。降钙素诱导这些多核细胞的胞质收缩，这表明它们类似于破骨细胞。培养7天后，猫骨髓培养物含有未附着的单核细胞和附着的单核细胞和多核细胞。我们从第7天开始分别培养贴壁和非贴壁细胞群，并在两种培养系统中观察到多核细胞的出现。地塞米松（10 - 9 ~ 10 -7 mol/L）可使两种培养液中多核细胞数减少。地塞米松的这种作用在来自非附着细胞的培养物中（7天）比在来自附着细胞的培养物中（14天）更快，这表明涉及两种不同的机制。地塞米松对已经形成的多核细胞的存活没有影响。
Our observations confirm the previously reported observation that multinucleated cells develop within a two-week period in cat bone-marrow cultures. Calcitonin induces cytoplasmic contraction in these multinucleated cells, which indicates that they resemble osteoclasts. After seven days of culture, cat bone-marrow cultures contain both non-attached mononuclear cells and attached mononuclear and polynuclear cells. We have cultured the attached and non-attached cell populations separately from day 7 onward and have observed the appearance of multinucleated cells in both culture systems. In both types of cultures, dexamethasone (10 -9-10-7 mol/L) reduced the number of multinucleated cells. This effect of dexamethasone was more rapid in the cultures derived from non-attached cells (seven days) than in cultures derived from attached cells (14 days), suggesting that two different mechanisms are involved. Dexamethasone had no effect on the survival of multinucleated cells already formed.