On the design and efficacy assessment of self-assembling peptide-based hydrogel-glycosaminoglycan mixtures for potential repair of early stage cartilage degeneration
On the design and efficacy assessment of self-assembling peptide-based hydrogel-glycosaminoglycan mixtures for potential repair of early stage cartilage degeneration
复制标题
DOI：
10.1002/psc.3114
复制
发表时间：
2018-08-01
影响因子：
2.1
通讯作者：
Davies, R. P. W.
中科院分区：
文献类型：
--
作者：
Barco, A.;Ingham, E.;Davies, R. P. W.
肽基水凝胶因其在再生医学中的潜在用途而受到关注。将这些水凝胶与可以增强其物理和生物特性的材料（例如糖胺聚糖）结合，具有扩展其生物医学应用范围的潜力，例如在早期软骨退化的修复中。本研究的目的是将三种自组装肽（P-11-4、P-11-8和P-11-12）与硫酸软骨素以1：16和1：64的两种摩尔比在130和230 mM Na+盐浓度下组合。该研究调查了自组装肽和糖胺聚糖在37 ℃下混合对物理和机械性能的影响。单独的肽，单独的硫酸软骨素，和肽与硫酸软骨素的组合进行了分析，使用傅里叶变换红外光谱法，以确定-片的百分比，透射电子显微镜，以确定原纤维形态，和流变学，以确定材料的弹性和粘性模量。所有变量（肽、盐浓度和硫酸软骨素摩尔比）都对水凝胶的机械性能、片层形成和原纤维形态产生影响。当与P-11-12/硫酸软骨素混合物相比时，两种摩尔比的P-11-4和P-11-8-硫酸软骨素混合物显示出在（130和230 mM）Na+盐溶液中具有高片层百分比、致密缠结的原纤维网络以及高机械刚度。这些肽/硫酸软骨素水凝胶显示出在糖胺聚糖耗尽组织中的生物医学应用的前景。
Peptide-based hydrogels are of interest for their potential use in regenerative medicine. Combining these hydrogels with materials that may enhance their physical and biological properties, such as glycosaminoglycans, has the potential to extend their range of biomedical applications, for example in the repair of early cartilage degeneration. The aim of this study was to combine three self-assembling peptides (P-11-4, P-11-8, and P-11-12) with chondroitin sulphate at two molar ratios of 1:16 and 1:64 in 130 and 230mM Na+ salt concentrations. The study investigates the effects of mixing self-assembling peptide and glycosaminoglycan on the physical and mechanical properties at 37 degrees C. Peptide alone, chondroitin sulphate alone, and peptide in combination with chondroitin sulphate were analysed using Fourier transform infrared spectroscopy to determine the -sheet percentage, transmission electron microscopy to determine the fibril morphology, and rheology to determine the elastic and viscous modulus of the materials. All of the variables (peptide, salt concentration, and chondroitin sulphate molar ratio) had an effect on the mechanical properties, -sheet formation, and fibril morphology of the hydrogels. P-11-4 and P-11-8-chondroitin sulphate mixtures, at both molar ratios, were shown to have a high -sheet percentage, dense entangled fibrillar networks, as well as high mechanical stiffness in both (130 and 230mM) Na+ salt solutions when compared with the P-11-12/chondroitin sulphate mixtures. These peptide/chondroitin sulphate hydrogels show promise for biomedical applications in glycosaminoglycan depleted tissues.