Fluorescence Lifetime Imaging in Retinal Artery Occlusion
Fluorescence Lifetime Imaging in Retinal Artery Occlusion
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DOI：
10.1167/iovs.14-16203
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发表时间：
2015-05-01
影响因子：
4.4
通讯作者：
Zinkernagel, Martin S.
中科院分区：
文献类型：
--
作者：
Dysli, Chantal;Wolf, Sebastian;Zinkernagel, Martin S.
目的.荧光寿命成像检眼镜是一种测量内源性视网膜荧光团衰减时间的技术。本研究的目的是探讨荧光寿命的眼睛与中央和分支视网膜动脉阻塞。24例中心或分支视网膜动脉阻塞患者纳入本研究。对侧未受影响的对侧眼用作对照。使用基于HRA Spectralis系统的荧光寿命成像检眼镜进行测量。荧光激发波长为473 nm，在短（498-560 nm）和长（560-720 nm）光谱通道中测量平均寿命。测量视网膜动脉阻塞区域的荧光寿命，并与对侧未受影响的眼睛的相应区域进行比较。此外，研究结果与光学相干断层扫描测量相关。本文分析了24例视网膜动脉阻塞患者的视网膜终身图像。与对侧未受影响的眼睛相比，在视网膜动脉阻塞后3天，缺血性视网膜区域的短光谱通道和长光谱通道中的平均视网膜荧光寿命分别延长了50%和20%。在急性期后的疾病阶段，受影响的地区和未受影响的对侧眼睛的寿命之间没有差异。视网膜动脉阻塞导致视网膜在急性期的荧光寿命显著延长，并可作为急性缺血性视网膜损伤的有用指标。
PURPOSE. Fluorescence lifetime imaging ophthalmoscopy is a technique to measure decay times of endogenous retinal fluorophores. The purpose of this study was to investigate fluorescence lifetimes in eyes with central and branch retinal artery occlusion.METHODS. Twenty-four patients with central or branch retinal artery occlusion were included in this study. The contralateral unaffected fellow eye was used as control. Measurements were performed using a fluorescence lifetime imaging ophthalmoscope based on a HRA Spectralis system. Fluorescence excitation wavelength was 473 nm, and mean lifetimes were measured in a short (498-560 nm) and in a long (560-720 nm) spectral channel. Fluorescence lifetimes in the area of retinal artery occlusion were measured and compared to corresponding areas in contralateral unaffected eyes. Additionally, findings were correlated to optical coherence tomography measurements.RESULTS. Retinal lifetime images of 24 patients with retinal artery occlusion were analyzed. Mean retinal fluorescence lifetimes were prolonged by 50% in the short and 20% in the long spectral channel in ischemic retinal areas up to 3 days after retinal artery occlusion compared to the contralateral unaffected eyes. In the postacute disease stage there was no difference between the lifetimes of affected areas and unaffected fellow eyes.CONCLUSIONS. Retinal artery occlusion leads to significantly longer fluorescence lifetimes of the retina in the acute phase and may serve as a useful indicator for acute ischemic retinal damage.