DIFFERENTIAL CONTRIBUTIONS OF OVARIAN AND EXTRAOVARIAN FACTORS TO AGE-RELATED REDUCTIONS IN PLASMA ESTRADIOL AND PROGESTERONE DURING THE ESTROUS-CYCLE OF C57BL/6J MICE
DIFFERENTIAL CONTRIBUTIONS OF OVARIAN AND EXTRAOVARIAN FACTORS TO AGE-RELATED REDUCTIONS IN PLASMA ESTRADIOL AND PROGESTERONE DURING THE ESTROUS-CYCLE OF C57BL/6J MICE
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DOI：
10.1210/en.130.2.805
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发表时间：
1992-02-01
期刊：
影响因子：
4.8
通讯作者：
FINCH, CE
中科院分区：
文献类型：
--
作者：
NELSON, JF;FELICIO, LS;FINCH, CE
卵巢和卵巢外因素的相对贡献，改变卵巢甾体分布的中年小鼠进行了评估，互惠，异时卵巢移植。通过在肾包膜下移植来交换来自循环、年轻（2个月）和中年（12个月）小鼠的卵巢。在整个发情周期中，每天中午采集血样用于测量雌二醇（E2），在发情前期熄灯后3-4 h采集血样用于测量排卵前孕酮（P4）的升高。与年轻小鼠相比，中年完整小鼠在周期中E2的平均浓度较低，未检测到中午排卵前E2升高，排卵前P4增加减弱。年轻供体的卵巢移植未能增加中年小鼠的平均E2水平，但确实使排卵前E2和排卵前P4的升高恢复到年轻对照组的水平。相互移植证实了这些发现：卵巢从中年捐助者在年轻的主机产生的平均E2水平相当于那些年轻的小鼠，但不能支持排卵前增加E2或排卵前P4水平相当于年轻的控制。这些结果揭示了卵巢和卵巢外因素对E2和P4年龄变化的不同贡献。他们指出，卵巢衰老在减弱排卵前E2和P4的增加中起着重要作用，但卵巢外，可能是神经内分泌，年龄变化的基础上，整个发情周期的E2水平的平均降低。
The relative contributions of ovarian and extra-ovarian factors to the altered ovarian steroidal profiles of middle-aged mice were assessed by reciprocal, heterochronic ovarian grafting. Ovaries from cycling, young (2 months), and middle-aged (12 months) mice were exchanged by grafting under the renal capsules. Blood samples were obtained daily at midday throughout the estrous cycle for measurement of estradiol (E2) and 3-4 h after lights-out on proestrus to measure the preovulatory elevation of progesterone (P4). Middle-aged intact mice had lower mean concentrations of E2 during the cycle, no detectable midday preovulatory elevation of E2, and an attenuated preovulatory increase of P4 compared to young mice. Ovarian grafts from young donors failed to increase mean E2 levels of middle-aged mice, but did restore the preovulatory elevation of E2 and preovulatory P4 to levels of young controls. Reciprocal grafting confirmed these findings: ovaries from middle-aged donors in young hosts produced mean E2 levels equivalent to those of young mice but were unable to support a preovulatory increase of E2 or a preovulatory P4 level equivalent to that of young controls. These results reveal differential contributions of ovarian and extra-ovarian factors to age changes in E2 and P4. They indicate that ovarian aging plays an important role in attenuating the preovulatory increase of E2 and P4, but extra-ovarian, presumably neuroendocrine, age changes underlie the mean reduction of E2 levels across the estrous cycle.