Magnetized Aerosols Comprising Superparamagnetic Iron Oxide Nanoparticles Improve Targeted Drug and Gene Delivery to the Lung
Magnetized Aerosols Comprising Superparamagnetic Iron Oxide Nanoparticles Improve Targeted Drug and Gene Delivery to the Lung
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DOI：
10.1007/s11095-012-0682-z
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发表时间：
2012-05-01
影响因子：
3.7
通讯作者：
Rudolph, Carsten
中科院分区：
文献类型：
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作者：
Hasenpusch, Guenther;Geiger, Johannes;Rudolph, Carsten
气雾剂的靶向给药不仅可以提高吸入药物的疗效，而且可以减少药物在健康组织中蓄积所引起的副作用。在这里，我们研究了磁化气溶胶对模型药物积累和转基因表达的影响，在磁靶向的非麻醉小鼠的肺部区域，含有超顺磁性氧化铁纳米粒子（SPION）和模型药物（荧光素或复合质粒DNA）的溶液雾化的影响下，外部磁场梯度指向肺部。随后在不同时间点测量药物积累和转基因表达。与未暴露于任何磁梯度的对照小鼠相比，在雾化期间暴露于外部磁梯度的小鼠的肺区域中模型药物荧光素和特异性转基因表达的积累高3倍。的药物和基因治疗剂，以治疗局部疾病的深呼吸道。
Targeted delivery of aerosols could not only improve efficacy of inhaled drugs but also reduce side effects resulting from their accumulation in healthy tissue. Here we investigated the impact of magnetized aerosols on model drug accumulation and transgene expression in magnetically targeted lung regions of unanesthetized mice.Solutions containing superparamagnetic iron oxide nanoparticles (SPIONs) and model drugs (fluorescein or complexed plasmid DNA) were nebulized to unanesthetized mice under the influence of an external magnetic gradient directed to the lungs. Drug accumulation and transgene expression was subsequently measured at different time points.We could demonstrate 2-3 fold higher accumulation of the model drug fluorescein and specific transgene expression in lung regions of mice which had been exposed to an external magnetic gradient during nebulization compared to the control mice without any exposure to magnetic gradient.Magnetized aerosols present themselves as an efficient approach for targeted pulmonary delivery of drugs and gene therapeutic agents in order to treat localized diseases of the deeper airways.