Binding of S21 to the 50S subunit and the effect of the 50S subunit on nonradiative energy transfer between the 3' end of 16S RNA and S21.
Binding of S21 to the 50S subunit and the effect of the 50S subunit on nonradiative energy transfer between the 3' end of 16S RNA and S21.
复制标题
S21 与 50S 亚基的结合以及 50S 亚基对 16S RNA 3 端和 S21 之间非辐射能量转移的影响。
DOI：
10.1021/bi00316a037
复制
发表时间：
1984
期刊：
影响因子：
2.9
通讯作者：
Hardesty,B
中科院分区：
文献类型：
--
作者：
Odom,OW;Deng,HY;Dabbs,ER;Hardesty,B
奥贝德·W放大图片作者：Eric R. Dabbs和Boyd Hardesty* 摘要：用荧光探针在其单个半胱氨酸基团处标记土方伊氏核糖体蛋白S21。标记的S21在支持甲酰基甲硫氨酰-tRNAf与30 S核糖体亚基的MS 2 RNA依赖性结合中显示出完全活性。荧光各向异性测量和甘油梯度的直接分析最终证明，标记的S21结合到50 S核糖体亚基以及30 S和70 S颗粒。相对结合亲和力在70 S量级
Obed W. Odom, Hung-Yin Deng, Eric R. Dabbs, and Boyd Hardesty* abstract: Escherichiacoli ribosomal protein S21 was labeled at its single cysteine group with a fluorescent probe. Labeled S21 showed full activity in supporting MS2 RNA-dependent binding of formylmethionyl-tRNAf to 30S ribosomal subunits. Fluorescence anisotropy measurements and direct analysis on glycerol gradients demonstrate conclusively that labeled S21 binds to 50S ribosomal subunits as well as to 30S and 70S particles. The relative binding affinities are in the order 70S