The effect of diet on protein concentration, hypopharyngeal gland development and virus load in worker honey bees (Apis mellifera L.)
The effect of diet on protein concentration, hypopharyngeal gland development and virus load in worker honey bees (Apis mellifera L.)
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DOI：
10.1016/j.jinsphys.2010.03.017
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发表时间：
2010-09-01
影响因子：
2.2
通讯作者：
Huang, Ming Hua
中科院分区：
文献类型：
--
作者：
DeGrandi-Hoffman, Gloria;Chen, Yanping;Huang, Ming Hua
阐明蜜蜂处理花粉与蛋白质补充剂的机制对于维持管理蜜蜂所需的人工饲料的产生至关重要。我们测量了饮食对工蜂蛋白质浓度、下咽腺发育和病毒滴度的影响，工蜂喂食花粉、蛋白质补充剂（MegaBee）或无蛋白质饮食的糖浆。工人消耗更多的花粉比蛋白质补充剂，但蛋白质量和下咽腺泡的大小没有显着差异。与其他饲喂处理相比，单独饲喂糖浆的蜜蜂具有较低的蛋白质浓度和较小的下咽腺，特别是随着蜜蜂的衰老。在试验开始时，在工人身上发现了畸形翅膀病毒。病毒浓度随着蜜蜂年龄的增长而增加，在喂食糖浆的蜜蜂中最高，在喂食花粉的蜜蜂中最低。总体结果表明饮食，蛋白质水平和免疫反应之间的联系，并表明通过补充饲料减轻蛋白质压力，可能会减少菌落损失。(C)2010年由Elsevier Ltd.出版
Elucidating the mechanisms by which honey bees process pollen vs. protein supplements are important in the generation of artificial diets needed to sustain managed honeybees. We measured the effects of diet on protein concentration, hypopharyngeal gland development and virus titers in worker honey bees fed either pollen, a protein supplement (MegaBee), or a protein-free diet of sugar syrup. Workers consumed more pollen than protein supplement, but protein amounts and size of hypopharyngeal gland acini did not differ between the two feeding treatments. Bees fed sugar syrup alone had lower protein concentrations and smaller hypopharyngeal glands compared with the other feeding treatments especially as the bees aged. Deformed wing virus was detected in workers at the start of a trial. The virus concentrations increased as bees aged and were highest in those fed sugar syrup and lowest in bees fed pollen. Overall results suggest a connection between diet, protein levels and immune response and indicate that colony losses might be reduced by alleviating protein stress through supplemental feeding. (C) 2010 Published by Elsevier Ltd.