PHYSICOCHEMICAL PROPERTIES OF MOUSE HEPATITIS VIRUS (MHV-2) GROWN ON DBT CELL-CULTURE
PHYSICOCHEMICAL PROPERTIES OF MOUSE HEPATITIS VIRUS (MHV-2) GROWN ON DBT CELL-CULTURE
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DOI：
10.1111/j.1348-0421.1978.tb00384.x
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发表时间：
1978-01-01
影响因子：
2.6
通讯作者：
FUJIWARA, K
中科院分区：
文献类型：
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作者：
HIRANO, N;HINO, S;FUJIWARA, K
在DBT细胞上生长的小鼠肝炎病毒MHV‐2株的一些特性是用细胞上的空斑试验测定的。放线菌素D或5 -碘- 2 -脱氧尿苷不抑制病毒生长。MHV‐2被乙醚、氯仿、脱氧胆酸钠或β‐丙内酯完全灭活，但对胰蛋白酶表现出中度抗性。56℃加热30分钟不能完全消除病毒的传染性。在1m‐mgcl2或1m‐mgso4中，在50℃下加热15分钟，以及在37℃下，在pH 3.0至9.0下加热60分钟后，病毒是稳定的。4℃保存30天和37℃保存24小时后，感染率分别降至1/100和1/400。病毒通过了200 nm而不是50 nm的赛氏膜过滤器。MHV‐2的浮力密度为1.183 g/cm3in蔗糖梯度，含有直径为70 ~ 130 nm的冠状病毒样颗粒。150ergs /mm2紫外线照射后存活率为10%。冷冻和解冻或在20 kc下超声波3分钟对病毒滴度没有影响。在鸡、日本鹌鹑、小鼠、大鼠、仓鼠、豚鼠、羊、牛或人的红细胞中均未发现血凝素。
Some properties of a strain of mouse hepatitis virus, MHV‐2, grown on DBT cells were determined using a plaque assay on the cells. Viral growth was not inhibited by the presence of actinomycin D or 5‐iodo‐2‐deoxyuridine. MHV‐2 was completely inactivated by ether, chloroform, sodium deoxycholate or beta‐propiolactone, but showed a moderate resistance to trypsin. Heating at 56 C for 30 min did not completely abolish the virus infectivity. The virus was stable after heating at 50 C for 15 min in 1m‐MgCl2or 1m‐MgSO4as well as at 37 C for 60 min at pH 3.0 to 9.0. Infectivity was decreased to 1/100 and 1/400 after storing at 4 C for 30 days and 37 C for 24 hr, respectively. The virus passed through a 200‐nm but not a 50‐nm Sartorius membrane filter. The buoyant density of MHV‐2 was 1.183 g/cm3in sucrose gradient, and the fraction contained coronavirus‐like particles measuring 70 to 130 nm in diameter. Survival rate was 10% after exposure to ultraviolet at 150 ergs/mm2. Freezing and thawing or sonication at 20 kc for 3 min did not affect the virus titer. No hemagglutinin was demonstrable with red blood cells of the chicken, Japanese quail, mouse, rat, hamster, guinea pig, sheep, bovine or human.