Efficient unwinding of triplex DNA by a DNA helicase.
Efficient unwinding of triplex DNA by a DNA helicase.
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通过 DNA 解旋酶有效解旋三链体 DNA。
DOI：
10.1006/bbrc.1994.2578
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发表时间：
1994
影响因子：
3.1
通讯作者：
Kodadek,T
中科院分区：
文献类型：
--
作者：
Maine,IP;Kodadek,T
序列特异性三螺旋形成作为一种通过阻断启动子或增强子来基因特异性抑制基因表达的策略显示出很好的前景。因此，了解这种不寻常的结构如何影响转录以外的DNA代谢过程是很重要的。已有研究表明，三联体在体外阻断了纯化的DNA聚合酶催化的DNA合成。我们在这里报道，纯化的DNA解旋酶解开三螺旋底物的效率与观察到的类似双链物种的效率相似。这些模型研究表明，三螺旋结构不会严重抑制体内的DNA复制或重组，因为DNA聚合酶之前是完全组装的复制全酶中的螺旋酶。
Sequence specific triple helix formation shows promise as a strategy for gene-specific inhibition of gene expression by blocking promoters or enhancers. Therefore, it is important to understand how this unusual structure affects DNA metabolic processes other than transcription. It has been shown that triplexes block in vitro DNA synthesis catalyzed by purified DNA polymerases. We report here that a purified DNA helicase unwinds a triple helical substrate with an efficiency similar to that observed with a comparable duplex species. These model studies suggest that triple helices will not seriously inhibit DNA replication or recombination in vivo, since DNA polymerases are preceded by helicases in the fully assembled replication holoenzyme.