Full‐field optical multi‐functional angiography based on endogenous hemodynamic characteristics
Full‐field optical multi‐functional angiography based on endogenous hemodynamic characteristics
复制标题
基于内源性血流动力学特征的全视场光学多功能血管造影
DOI：
10.1002/jbio.202000411
复制
发表时间：
2021
影响因子：
2.8
通讯作者：
YaguangZeng
中科院分区：
文献类型：
--
作者：
CaizhongGuan;MinYi;QianyiDu;HonglianXion;HaishuTan;MingyiWang;YaguangZeng
血流功能成像技术广泛应用于生物学研究中，可以提供血管的形态学和统计学参数。它依赖于吸收差异，因此容易受到复杂生物结构的影响，无法容纳丰富的功能信息。我们提出了一种全视野多功能血管造影方法来分类动静脉血管，并同时显示流速和血管直径分布。与以前的方法不同，使用欠采样激光多普勒采集模式来记录低相干散斑，并通过调制低相干散斑的内源性血流动力学特征来实现多功能血管造影。为了展示使用我们的方法实现的分类血管造影、血流速度测量和血管直径测量的组合，我们对流动体模和活鸡胚进行了实验，并生成了多功能血管造影照片。所提出的方法可用作无标记多功能血管造影技术，其中红细胞提供了天然血流动力学特征的强大内源性来源。
Blood flow functional imaging is widely applied in biological research to provide vascular morphological and statistical parameters. It relies on the absorption difference and is, therefore, easily affected by complex biological structures, and it cannot accommodate abundant functional information. We propose a full‐field multi‐functional angiography method to classify arteriovenous vessels and to display flow velocity and vascular diameter distribution simultaneously. Unlike previous methods, an under‐sampled laser Doppler acquisition mode is used to record the low‐coherence speckle, and multi‐functional angiography is achieved by modulating the endogenous hemodynamic characteristics from low‐coherence speckle. To demonstrate the combination of classified angiography, blood flow velocity measurement, and vascular diameter measurement realized using our method, we performed experiments on the flow phantom and living chicken embryos and generated multi‐functional angiograms. The proposed method can be used as a label‐free multi‐functional angiography technique in which red blood cells provide a strong endogenous source of naturally hemodynamic characteristics.