Celastrus orbiculatus Thunb. Decreases Athero-Susceptibility in Lipoproteins and the Aorta of Guinea Pigs Fed High Fat Diet
Celastrus orbiculatus Thunb. Decreases Athero-Susceptibility in Lipoproteins and the Aorta of Guinea Pigs Fed High Fat Diet
复制标题
南蛇藤 Thunb.
DOI：
10.1007/s11745-013-3773-7
复制
发表时间：
2013-03
期刊：
影响因子：
1.9
通讯作者：
Wang, Jianhua
中科院分区：
文献类型：
--
作者：
Qin, Shucun;Si, Yanhong;Yao, Shutong;Yang, Nana;Song, Guohua;Sang, Hui;Zu, D;an;Xu, Xin;Wang, Jianhua
南蛇藤（Celastrus orbiculatus Thunb.，COT）是一种传统中药材，具有抗炎和抗氧化特性。在本研究中，我们检测了南蛇藤对高脂肪饮食诱导的动脉粥样硬化起始的保护作用，并探索了其潜在机制（此处“und”可能是“underlying mechanism”未写完的部分）。
Celastrus orbiculatus Thunb. (COT), a traditional Chinese herb, has anti‐inflammatory and anti‐oxidative properties. In this study, we examined the protective effect of COT on the initiation of atherosclerosis induced by high fat diet and explored the underlying mechanisms. We established guinea pig models of hyperlipidemia and treated them with three dosages of COT or 20 mg/kg/d simvastatin (a positive control drug) for 8 weeks. Plasma lipid analysis indicated that COT decreased total cholesterol (TC), non‐high‐density lipoprotein cholesterol (non‐HDL‐C), triglyceride (TG), apolipoprotein B100 (apoB100) and apolipoprotein E (apoE) levels and increased high density lipoprotein cholesterol (HDL‐C) level. The analysis of the hepatic gene involving cholesterol metabolism by quantitative real‐time PCR revealed that COT upregulated the mRNA abundance of LDL receptor (LDL‐R), scavenger receptor class B type 1 (SR‐B1), cholesterol 7α‐hydroxylase A1 (CYP7A1) and the 3‐hydroxy‐3‐methyl‐glutaryl‐CoA reductase (HMGCR). Oil red O staining showed COT significantly reduced lipid deposition in the arterial wall. Moreover, ELISA assay revealed COT lowered the levels of C‐reactive protein (CRP), interleukin‐6 (IL‐6), and tumor necrosis factor‐α (TNF‐α) in plasma. Meanwhile, the level of Nitric oxide (NO) in plasma was increased by COT. Immunohistochemistry and Western blot analysis showed the expression of CD68 and active NF‐kB p65 proteins in the arterial wall was decreased by COT. The content of Malondialdehyde (MDA) and activity of Superoxide dismutase (SOD) in plasma were determined and the data indicated COT suppressed oxidative stress reaction. These results reveal that administration of COT decreases athero‐susceptibility through lowering plasma lipid, attenuating inflammation, and suppressing oxidative stress in guinea pig fed high fat diet.
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DOI：
10.1016/j.atherosclerosissup.2004.08.024
发表时间：
2004-10
期刊：
Atherosclerosis. Supplements
影响因子：
--
作者：
M. Brown;J. Goldstein
通讯作者：
M. Brown;J. Goldstein
影响因子：
1.9
作者：
D. Grove;H. Pownall
通讯作者：
D. Grove;H. Pownall
DOI：
10.1046/j.1432-1327.1998.2520378.x
发表时间：
1998-03
期刊：
European journal of biochemistry
影响因子：
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作者：
F. Vidal;C. Colomé;J. Martínez-González;L. Badimón
通讯作者：
F. Vidal;C. Colomé;J. Martínez-González;L. Badimón
DOI：
10.1016/s1062-1458(01)00374-9
发表时间：
2001-05
期刊：
JAMA
影响因子：
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作者：
P. Ridker;M. Stampfer;N. Rifai
通讯作者：
P. Ridker;M. Stampfer;N. Rifai
DOI：
10.1111/j.1527-3466.2004.tb00131.x
发表时间：
2006-06
期刊：
Cardiovascular drug reviews
影响因子：
--
作者：
K. L. West;M. Fernández
通讯作者：
K. L. West;M. Fernández