Spatial Arrangement of Glomerular Molecular-Feature Clusters in the Odorant-Receptor Class Domains of the Mouse Olfactory Bulb
Spatial Arrangement of Glomerular Molecular-Feature Clusters in the Odorant-Receptor Class Domains of the Mouse Olfactory Bulb
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DOI：
10.1152/jn.00035.2010
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发表时间：
2010-06-01
影响因子：
2.5
通讯作者：
Mori, Kensaku
中科院分区：
文献类型：
--
作者：
Matsumoto, Hideyuki;Kobayakawa, Ko;Mori, Kensaku
松本H，Kobayakawa K，Kobayakawa R，Tashiro T，Mori K，Sakano H，Mori K.小鼠嗅球气味受体类结构域中小球分子特征簇的空间排列。神经生理学杂志103：3490-3500，2010年。首次发表于2010年4月14日; doi：10.1152/jn.00035.2010。哺乳动物嗅球（OB）的肾小球层形成气味受体（OR）图谱。每个OR图在结构和功能上划分为区域（背侧和腹侧）和域（背侧区域中的DI和DII）。我们以前报道，肾小球具有类似的分子感受范围的性质形成的分子特征集群在刻板的位置在大鼠OB。然而，关于OR区和域的分子特征簇的空间排列尚未被系统地研究。在这项研究中，我们光学映射的分子特征集群的肾小球内的域和区域框架的OB使用域可见的II类GFP转基因小鼠。在所有的小鼠检查，脂肪酸反应集群A位于域DI的外侧部分，而集群B，C和D在域DII内的前向后顺序排列。我们还发现了一个新的肾小球簇，对狐狸气味三甲基噻唑啉及其结构类似物（环内含有硫和氮原子的杂环气味剂）作出反应。该簇（命名为簇J）位于DII结构域内簇D的后面。这些结果表明，分子特征簇对应于特定的肾小球子集在选择性结构域的OR地图，这表明分子特征簇代表特定的OR，具有相似的分子感受范围特性和功能作用。
Matsumoto H, Kobayakawa K, Kobayakawa R, Tashiro T, Mori K, Sakano H, Mori K. Spatial arrangement of glomerular molecular-feature clusters in the odorant-receptor class domains of the mouse olfactory bulb. J Neurophysiol 103: 3490-3500, 2010. First published April 14, 2010; doi:10.1152/jn.00035.2010. The glomerular layer of the mammalian olfactory bulb (OB) forms odorant receptor (OR) maps. Each OR map is structurally and functionally compartmentalized into zones (dorsal and ventral) and domains (DI and DII in the dorsal zone). We previously reported that glomeruli with similar molecular receptive range properties formed molecular feature clusters at stereotypical positions in the rat OB. However, the spatial arrangement of the molecular feature clusters with regard to the OR zones and domains has not been systematically examined. In this study, we optically mapped the molecular feature clusters of glomeruli within the domain and zone framework of the OB using domain-visible class II GFP transgenic mice. In all mice examined, fatty acid-responsive cluster A was located in the lateral part of domain DI, whereas clusters B, C, and D were arranged in an anterior to posterior order within domain DII. We also found a new cluster of glomeruli that respond to fox odor trimethyl-thiazoline and its structural analogs (heterocyclic odorants that contain sulfur and nitrogen atoms within the ring). This cluster (named cluster J) was located posterior to cluster D within the DII domain. These results show that molecular feature clusters correspond to specific subsets of glomeruli in selective domains of the OR map, suggesting that the molecular feature clusters represent specific ORs that have similar molecular receptive range properties and functional roles.