HISTOCHEMICAL-CHANGES IN CYTOCHROME-OXIDASE OF CORTICAL BARRELS AFTER VIBRISSAL REMOVAL IN NEONATAL AND ADULT MICE
HISTOCHEMICAL-CHANGES IN CYTOCHROME-OXIDASE OF CORTICAL BARRELS AFTER VIBRISSAL REMOVAL IN NEONATAL AND ADULT MICE
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DOI：
10.1073/pnas.77.4.2333
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发表时间：
1980-01-01
期刊：
影响因子：
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通讯作者：
WELT, C
中科院分区：
文献类型：
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作者：
WONGRILEY, MTT;WELT, C
通过组织化学检查小鼠体感皮层后内侧桶状亚区的细胞色素氧化酶活性（细胞色素c氧化酶；铁细胞色素c：氧氧化还原酶，EC 1.9.3.1）。在正常小鼠中，在桶状空腔内发现了高酶活性，而不是在侧面和隔膜中。电镜检查表明，在空洞内，反应性线粒体存在于许多树突、一些轴突末端和一些神经元周核中。在对选定的触须行进行新生儿烧灼后，相应的桶行显示为狭窄的融合带，并且它们的细胞色素氧化酶活性大大降低。通过烧灼或反复拔毛去除成人的触须，不会导致皮质桶的大小发生变化。这些桶内的氧化酶活性显着降低。因此，通过损伤或去除外周感觉器官而剥夺感觉输入会在距外周至少 2-3 个突触的神经元中诱导酶反应。
The posteromedial barrel subfield of the somatosensory cortex of mice was examined histochemically for cytochrome oxidase activity (cytochrome c oxidase; ferrocytochrome c:oxygen oxidoreductase, EC 1.9.3.1). In normal mice, a high enzymatic activity was found within the barrel hollows, rather than in the sides and septa. EM examination indicated that within the hollows reactive mitochondria reside in many dendrites, in some axonal terminals and in a few neuronal perikarya. After neonatal cauterization of selected row(s) of vibrissae, the corresponding row(s) of barrels appeared as narrowed fused band(s) and their cytochrome oxidase activity was much reduced. Removal of vibrissae in the adult, by either cauterization or repeated plucking, did not cause size changes of cortical barrels. There was a significant decrease in the oxidative enzymatic activity within these barrels. Thus, the deprivation of sensory input through damage to, or removal of the peripheral sensory organ induces an enzymatic response in neurons that are at least 2-3 synapses away from the periphery.