Sex and species differences in mouse and rat forebrain commissures depend on the method of adjusting for brain size
Sex and species differences in mouse and rat forebrain commissures depend on the method of adjusting for brain size
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DOI：
10.1016/s0006-8993(98)01088-9
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发表时间：
1999-01-09
期刊：
影响因子：
2.9
通讯作者：
Wahlsten, D
中科院分区：
文献类型：
--
作者：
Bishop, KM;Wahlsten, D
在B6D2F(2)杂交鼠和SpragueDawley大鼠中，观察了前脑连合(胼胝体、海马连合和前连合)的性别差异。分别于21日龄、42日龄和63日龄分别对24只雄性和雌性产仔小鼠进行灌流，从成块染色的组织中测量连合的矢状面正中面积。用同样的方法对110日龄的22对雄性和雌性产仔大鼠进行了检查。雄性小鼠的身体比雌性大，但在小鼠的大脑重量和连合面积方面没有性别差异。相反，在大鼠的身体、脑、胼胝体和海马区连合的大小上发现了显著的性别差异。四种方法被用来调整大脑大小的差异(比率、几何、线性回归和异速生长)。当对这两个物种分别进行分析时，如果进行回归或异速生长调整，小鼠和大鼠在与脑大小相关的连合区域中都没有显示出显著的性别差异。即使将小鼠和大鼠的数据合并为一个具有广泛数值范围的大组，在使用回归或异速生长方法对大脑大小进行调整后，也没有明显的物种或性别差异。不建议使用比率来调整总体大小的差异，特别是因为这种方法不能有效地消除脑大小与联合大小的影响；比率和脑大小之间往往存在显著的相关性。(C)1999 Elsevier Science B.V.保留所有权利。
Sex differences in the forebrain commissures (corpus callosum, hippocampal commissure, and anterior commissure) were examined in B6D2F(2) hybrid mice and Sprague-Dawley rats. Twenty-four male-female Littermate pairs of mice were perfused at each of 21, 42 and 63 days of age and the midsagittal area of the commissures was measured from en bloc stained tissue. Twenty-two male-female littermate pairs of rats were examined at 110 days of age using the same methods. Male mice had larger bodies than females but no sex differences were found for mouse brain weight or commissure areas. In contrast, a significant sex difference was found for rat body, brain, corpus callosum and hippocampal commissure sizes. Four methods were used to adjust for differences in brain size (ratio, geometric, linear regression, and allometric). When the two species were analysed separately, neither mice nor rats showed significant sex differences in commissure areas relative to brain size if regression or allometric adjustments were made. Even when data from mice and rats were combined into one large group with a wide range of values, no species or sex differences were apparent after adjustments were made for brain size with either the regression or allometric methods. The use of ratios to adjust for differences in overall size is not recommended, especially because this method does not effectively remove the influence of brain size from commissure size; a substantial correlation is often present between the ratio and brain size. (C) 1999 Elsevier Science B.V. All rights reserved.