Cloning and expression of Na+/K+-ATPase and osmotic stress transcription factor 1 mRNA in black porgy, Acanthopagrus schlegeli during osmotic stress
Cloning and expression of Na+/K+-ATPase and osmotic stress transcription factor 1 mRNA in black porgy, Acanthopagrus schlegeli during osmotic stress
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DOI：
10.1016/j.cbpb.2007.08.009
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发表时间：
2008-01-01
影响因子：
2.2
通讯作者：
An, Kwang Wook
中科院分区：
文献类型：
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作者：
Choi, Cheol Young;An, Kwang Wook
从黑鲷肾脏和鳃中分别克隆了Na+/K+-ATP酶（NKA）和渗透胁迫转录因子1（OSTF 1）的cDNA。黑鲷NKA全长cDNA由3078 bp组成，编码1025个氨基酸; OSTF 1部分cDNA由201 bp组成。为了研究黑鲷在转移到淡水中时的摄食调节能力，我们检测了黑鲷摄食调节器官中NKA和OSTF 1 mRNA的表达，鳃、肾和肠，采用定量聚合酶链反应（QPCR）。为了确定NKA和OSTF 1诱导的低渗应激特异性，将黑鲷暴露于30 ℃水温24 h。在鳃中，NKA mRNA在FW中的表达量是对照组的4.2倍，在肾脏中的表达量是对照组的5.7倍相比之下，鳃中的OSTF 1 mRNA在FW中是SW中的3.7倍。热休克蛋白90（HSP 90）mRNA的表达不仅发生在向FW转移的过程中，而且在所有测试组织的高温水中也是如此，尽管mRNA水平没有显著差异。从SW到FW，血浆渗透压降低，皮质醇水平升高。这些结果表明，NKA和OSTF 1基因在高渗调节器官的激素调节中起重要作用，并且这些基因是低渗胁迫所特有的，提高了黑鲷在低渗环境中的高渗调节能力。(C)2007年爱思唯尔公司All rights reserved.
We cloned complementary DNA (cDNA) encoding the Na+/K+-ATPase (NKA) and the osmotic stress transcription factor 1 (OSTF1) from the kidney and gill, respectively, of the black porgy, Acanthopagrus schlegeli. Black porgy NKA full-length cDNA consists of 3078 base pairs (bp) and encodes a protein of 1025 amino acids; OSTF1 partial cDNA consists of 201 bp. To investigate the osmoregulatory ability of black porgy when black porgy were transferred to freshwater (FW), we examined the expression of NKA and OSTF1 mRNA in osmoregulatory organs, i.e., gill, kidney and intestine, using quantitative polymerase chain reaction (QPCR). To determine the hypoosmotic stressor specificity of the induction of NKA and OSTF1, black porgy were exposed to 30 degrees C water temperature for 24 h. In the gill, NKA mRNA was 4.2 times higher in FW, its expression in the kidney was 5.7 times higher in 10 parts per thousand seawater (10 parts per thousand SW) than in SW. In contrast, OSTF1 mRNA in the gill was 3.7 times higher in FW than in SW. The expression of heat shock protein 90 (HSP90) mRNA occurred not only during transfer to FW, but also in high-temperature water in all tested tissues, although the mRNA levels were not significantly different. Plasma osmolality level was decreased and cortisol level was increased when the fish were transferred from SW to FW. These results suggest that NKA and OSTF1 genes play important roles in hormonal regulation in osmoregulatory organs and that these genes are specific to hypoosmotic stress, improving the hyperosmoregulatory ability of black porgy in hypoosmotic environments. (C) 2007 Elsevier Inc. All rights reserved.